Methanol (HPLC grade) and (E)-3-hexen-1-ol (≥98%) was purchased from Sigma Aldrich (Milan, Italy). Ten milliliters headspace vials with magnetic screw cap containing a pierceable PTFE/silicon septa were purchased from Agilent Technologies (Palo Alto, CA, USA). Helium at a purity of 99.9995% was obtained by Sapio s.r.l. (Bari, Italy). The automatic solid-phase microextraction (SPME) fiber holder was obtained from Gerstel (Mulheim an der Ruhr, Germany). SPME-Fast Fit Fiber Assembly (FFA) divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS, 50/30 μm, 1 cm fiber length), SPME Fiber Assembly for manual use divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS, 50/30 μm, 1 cm fiber length), polydimethylsiloxane/divinylbenzene (PDMS/DVB, 65 μm, 1 cm fiber length), carboxen/polydimethylsiloxane (CAR/PDMS, 85 μm, 1 cm fiber length) and the manual SPME holder were purchased from Supelco (Bellafonte, PA, USA).
Manual spme holder
Manufactured by Merck Group
Sourced in United States
The Manual SPME holder is a device used to hold and manipulate solid-phase microextraction (SPME) fibers. It is a manual tool that allows users to manually insert, expose, and retract SPME fibers during sample preparation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Dvb car pdms, by Merck Group (2 mentions)
Car pdms fiber, by Merck Group (2 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Carboxen polydimethylsiloxane, by Merck Group (1 mentions)
E 3 hexen 1 ol, by Merck Group (1 mentions)
Polydimethylsiloxane divinylbenzene, by Merck Group (1 mentions)
Turbomass, by PerkinElmer (1 mentions)
Agilent 7890 gas chromatograph, by Agilent Technologies (1 mentions)
Cyclohexane, by Sinopharm (1 mentions)
Khco3, by Sinopharm (1 mentions)
Pcb 52, by AccuStandard (1 mentions)
Acetone, by AccuStandard (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Methanol, by Scharlab (1 mentions)
13 protocols using manual spme holder
1
Headspace Solid-Phase Microextraction Analysis
2
Headspace SPME Analysis of Volatiles
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A manual SPME holder with carboxen/polydimethylsiloxane (CAR/PDMS) fibers with a thickness of 75 µm was purchased from Supelco (Bellefonte, USA). The SPME fibers were inserted into the vials and exposed to the gaseous samples for 20 min at 40 °C. Subsequently, desorption of volatiles was performed in a hot GC injector at 200 °C for 2 min.
3
Profiling Volatile Metabolites from Coprolites
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The experimental design was modified from that of Brown et al.14 (link) Coprolites and sediments were crushed using a mortar and pestle and then passed through a 2 mm sieve. Approximately 0.05 g of each sample was placed into a 10 mL headspace vial with PTFE/silicone septa lid (Fisher Scientific, UK) and five drops of double distilled water were added to accelerate the release of VOCs.53 (link) The sample was then placed in a heating block and, maintained at a temperature of 60°C for 1 h. After sample incubation, a solid-phase microextraction (SPME) fibre (50/30 μm DVB/CAR/PDMS; Supelco, Bellefonte, USA) in a manual SPME holder (Supelco, USA) was used to sample the head-space air above faeces for 20 min. The SPME fibre was conditioned in injector of the gas chromatograph (GC) at 250°C for at least one hour prior to each sampling event, thereby ensuring that no interfering peaks were obtained. The fibre was then retracted and immediately (<1 min) manually injected into the injection port of a GC/Q-TOFMS for characterisation.
4
Headspace SPME-GC-MS Analysis of Fire Debris
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The ILRs of the samples were first extracted using a headspace concentration-solid phase microextraction (HS-SPME) procedure. A 65 μm polydimethylsiloxane-divinylbenzene (PDMS/DVB) fibre housed in a manual SPME holder (Supelco, Bellefonte, PA, USA) was used for the extraction. To ensure no component peak arose from the SPME membrane, the fibre blank was first introduced into the GC–MS system and analysed according to the method used for analysing ILRs extracted from the fire debris samples. Given a fire debris sample, headspace was first created by keeping it in an oven at 90°C for 15 min. Then, the nylon bag was removed from the oven and subsequently pierced with the SPME needle. After 20 min, the SPME needle was removed and directly inserted into the gas chromatographic injector port. An SPME fibre blank was performed each time before a new sample injection to ensure no carryover of the previous sample.
All the GC–MS analyses were performed on a PerkinElmer Clarus® 680 gas chromatography coupled with a Clarus® SQ 8T mass spectrometry (Waltham, MA, USA).Table 1 presents the parameters adopted to perform the GC–MS analysis. The GC–MS data were acquired by a PerkinElmer TurboMass (version 6.1.0).
All the GC–MS analyses were performed on a PerkinElmer Clarus® 680 gas chromatography coupled with a Clarus® SQ 8T mass spectrometry (Waltham, MA, USA).
5
HS-SPME Analysis of Volatile Compounds
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HS-SPME analysis was performed by using a 75 μm carboxen-polydimethylsiloxane (CAR-PDMS) SPME fiber equipped with a manual SPME holder (Supelco, Bellefonte, PA, USA). Samples were equilibrated for 20 min at room temperature before analysis and the SPME fiber was conditioned at the gas chromatographic injection port for 40 min at 250 °C before the first volatile collection. After that, the fiber was inserted into the headspace of the capped vial with SPME holder to absorb volatile compounds for 40 min at 45 °C (water bath). The empty capped vial was used as the blank control.
6
Volatile Organic Compound Profiling of Virus-Infected Plants
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Ten healthy, mock-inoculated or MMWV-infected plants (10 days after inoculation) were placed in a glass desiccator (36 L). Plants were illuminated with fluorescent bulbs (50 μmol m−2 s−1 with a photoperiod of 16 h light: 8 h dark). The temperature in the glass desiccators was 25 °C, and the relative humidity was 70%. The Pots with potting soil were wrapped with aluminum foil to prevent water evaporation and volatile emission from the soil. A watered pot wrapped in aluminum foil without plants was used as a negative control. The glass desiccator was connected to a GC-grade air generator (QL-3, Shandong Saikesaisi Hydrogen Energy Co., Ltd., Shandong, China) through a cork plug with two openings allowing gases to go in and out at a flow rate of 350 ml min−1. A clean Pasteur glass pipette was inserted in the outlet of the cock plug and VOCs were collected with divinyl-benzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS, 50 μm/30 μm, No.57348-U) solid-phase microextraction (SPME) fibers (Bellefonte, PA, USA). The manual SPME holder was purchased from Supelco (Bellefonte, PA, USA) (Figure 1 ). SPME fibers were conditioned at 250 °C for 1 h, and then placed in the Pasteur glass pipette to absorb VOCs for 2 h. All assays were performed in triplicate.
7
Headspace SPME Sampling and Analysis
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A manual SPME holder with 75-um-thick carboxyl/polydimethylsiloxane (CAR/PDMS) bers was purchased from Supelco (Bellefonte, USA). The SPME ber was inserted into a vial and exposed to a gas sample at 40°C for 20 minutes. Subsequently, the SPME holder was placed in the gas chromatograph inlet for desorption.
8
Headspace Sampling of Volatile Compounds
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A manual SPME holder with carboxen/polydimethylsiloxane (CAR/PDMS) fibers of 75 µm thickness was purchased from Supelco (Bellefonte, USA). The SPME fiber was inserted into the vial and exposed to the gaseous sample for 20 min at 40°C. Subsequently, the desorption of volatiles occurred in the hot GC injector at 200°C for 2 min.
9
GC-MS Analysis of Milk Volatiles
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Volatile compounds for each milk sample were evaluated by GC-MS/solid-phase microextraction (SPME) analysis as previously described (Lanciotti et al., 2006) . A divinylbenzenecarboxen-polydimethylsiloxane-coated fibre (65 µm) and a manual SPME holder (Supelco) were used for the SPME of volatile compounds in milk. For peak detection, an Agilent 7890 gas chromatograph (Agilent Technologies, Santa Clara, FL, USA) coupled to an Agilent 5970 mass selective detector was used. This system was operated in electron impact mode with an ionisation voltage of 70 eV. The column used was a Chrompack CP-Wax 52 CB capillary column (50 m × 0.32 mm i.d.; Chrompack, Middetburg, Netherlands). The temperature was adjusted to 50 °C for 2 min and then raised 1 °C every minute up to 65 °C and after that 5 °C per min to 220 °C. The temperatures of the injector, interface, and ion source were 250, 250, and 230°C, respectively.
Injections were carried out in splitless mode, and the carrier gas was helium with a constant flow rate of 1 mL min -1 . Volatile compounds were identified using mass spectra databases (NIST/EPA/NIH version 2005). The quantification of the main volatile compounds was performed
7 on the basis of calibration curves obtained by adding pure standards to 5 mL of milk samples and prepared as previously described for volatile compound analysis.
Injections were carried out in splitless mode, and the carrier gas was helium with a constant flow rate of 1 mL min -1 . Volatile compounds were identified using mass spectra databases (NIST/EPA/NIH version 2005). The quantification of the main volatile compounds was performed
7 on the basis of calibration curves obtained by adding pure standards to 5 mL of milk samples and prepared as previously described for volatile compound analysis.
10
Headspace-SPME Extraction of Wine Aroma
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SPME manual holder and fused silica fiber coated with Carboxen ® /Polydimetilsiloxane (CAR/PDMS) stationary phase (85 µm thickness) were purchased from Supelco (Bellefonte, PA, USA) and used for the extraction of the aroma compounds from the wine samples by HS-SPME. Before the first use, the fiber was preconditioned according to the manufacturer's instruction. Twenty milliliters of the wine sample, 3 g of NaCl and a magnetic stirrer bar were placed in a 30 mL amber glass bottle, closed with rubber septum and sealed with parafilm. In order to achieve the equilibration state, the samples were heated at 55 • C and agitated with the magnetic stirrer for 15 min (pre-extraction). The SPME fiber was then inserted into the headspace of the sample and the volatiles were extracted for 35 min with constant heating and stirring at 55 • C. The fiber was than desorbed for 10 min in a split/splitless inlet set at 250 • C in 20:1 split mode and analyzed by GC/MS and GC/FID.
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