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Vinblastine

Manufactured by AG Scientific

Vinblastine is a chemical compound used in laboratory research. It is a mitotic inhibitor, which means it interferes with the process of cell division. Vinblastine is commonly used in cell biology and cancer research studies.

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2 protocols using vinblastine

1

Fibroblast and Cancer Cell Culture Protocols

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Human IMR-90 primary lung fibroblasts (ATCC) and their derivatives were grown in Glutamax-DMEM (Gibco) supplemented with 0.1 mM nonessential Amino Acids and 15% Fetal Bovine Serum. HT1080 6TG cells were grown in Glutamax-DMEM supplemented with 0.1 mM Nonessential Amino Acids and 10% Bovine Growth Serum. All cells were grown at 7.5% CO2 and 3% O2. Colcemid (Life Technologies), Taxol (A. G. Scientific), vinblastine (A. G. Scientific), dimethylenastron (A. G. Scientific), hesperadin (Selleck), reversine (Selleck) and camptothecin (Selleck) were used at indicated concentration. ATM inhibitor (KU-55933) (Tocris) was used at 10 μM. FACS analysis was performed as described27 . Cells were tested for mycoplasma contamination and found negative.
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2

Fibroblast and Cancer Cell Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human IMR-90 primary lung fibroblasts (ATCC) and their derivatives were grown in Glutamax-DMEM (Gibco) supplemented with 0.1 mM nonessential Amino Acids and 15% Fetal Bovine Serum. HT1080 6TG cells were grown in Glutamax-DMEM supplemented with 0.1 mM Nonessential Amino Acids and 10% Bovine Growth Serum. All cells were grown at 7.5% CO2 and 3% O2. Colcemid (Life Technologies), Taxol (A. G. Scientific), vinblastine (A. G. Scientific), dimethylenastron (A. G. Scientific), hesperadin (Selleck), reversine (Selleck) and camptothecin (Selleck) were used at indicated concentration. ATM inhibitor (KU-55933) (Tocris) was used at 10 μM. FACS analysis was performed as described27 . Cells were tested for mycoplasma contamination and found negative.
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