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2 protocols using orthophosphoric acid

1

Yoghurt Starter Culture and Probiotics

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A commercial yoghurt starter culture (YOFLEX®—consisting of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and three probiotic strains (Lacticaseibacillus rhamnosus LGG, Lacticaseibacillus casei 431, Bifidobacterium animalis subsp. lactis BB-12) were kindly supplied by Chr. Hansen, Bayswater, VIC, Australia. Powdered skim milk (Australian instant skim milk powder, Coles) was obtained from a local supermarket in Melbourne, VIC, Australia. Pancreatin was purchased from Alfa Aesar (Ward Hill, MA, USA). Mangiferin, pyrogallol, gallic acid, caffeic acid, 3-hydroxy phenyl acetic acid, 3-(2-hydroxyphenyl) propionic acid, porcine pepsin, α-amylase from aspergillus oryzae, p-nitrophenyl-α-d-glucopyranoside, bile salts, LC-MS grade formic acid, acetic acid, 4-methyl valeric acid, guar, casein, tryptone, cysteine HCl, peptone, mucin, pectin, potato starch, yeast extract, and MRS agar were acquired from Sigma–Aldrich (Castle Hill, NSW, Australia). Sodium carbonate (anhydrous), HCl, NaHCO3, NaOH, (NH4)2CO3, KCl, NaCl, KH2PO4, MgCl2(H2O)6, CaCl2, K2HPO4, Na3PO4, MgSO4.7H2O Tween 80, orthophosphoric acid, acetonitrile, and methanol of HPLC grade were acquired from Chem-supply Pty Ltd. (Melbourne, VIC, Australia).
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2

Graphite-Based Composite Synthesis

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Graphite flakes, diethylene glycol (DEG), hydrogen peroxide
(H2O2), poly (vinyl alcohol) (PVA, Mw:
146000 ~ 186000) and hypophosphorous acid
(HPA) were purchased from Sigma-Aldrich. Concentrated sulphuric acid
(H2SO4, 98%), orthophosphoric acid
(H3PO4, 85%), and hydrochloric acid (HCl, 32%) were
obtained from Chem-Supply.
Poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) pellets was
Orgacon™ DRY re-dispersible product from Agfa company.
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