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2 protocols using z devd fmk

1

Apoptosis and Necrosis Assay Protocol

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JC-1, Newport Green™ DCF diacetate, DQ-Green BSA and Click-iT EdU Kit were acquired from Molecular Probes, Inc. (Eugene, OR, USA). Zinc pyrithione, acridine orange, cyclosporin A, monodansylcadaverine (MDC), N-acetyl cysteine (NAC), 3-methyladenine (3-MA), chloroquine (CQ), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), horseradish peroxidase, Triton-X, dithiotreitol (DTT), bisBenzimide H 33342 trihydrochloride (Hoechst 33342) and 4’,6-Diamidino-2-Phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). WST-1 was purchased from Roche Diagnostics (Manheim, Germany). Caspase-3 inhibitor z-devd-fmk was from ICN Biomedicals Inc. (Irvine, CA, USA). Necrostatin-1 was from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Primary and secondary antibodies were from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were of the highest analytical grade.
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2

Comprehensive Cellular Analysis Toolkit

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Newport Green™ DCF diacetate, MitoSOX™ Red were acquired from Molecular Probes, Inc. (Eugene, OR, USA). TPEN (N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine), acridine orange, cyclosporin A, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), EdU (5-ethynyl-2′-deoxyuridine), horseradish peroxidase, Triton-X, dithiotreitol (DTT), propidium iodide and 4’,6-Diamidino-2-Phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). WST-1 was purchased from Roche Diagnostics (Manheim, Germany). Caspase-3 inhibitor z-devd-fmk was from ICN Biomedicals Inc. (Irvine, CA, USA). Primary and secondary antibodies were from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were of the highest analytical grade.
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