Ccd 18co normal colon fibroblasts

HCT116 colorectal cancer cell line and CCD-18Co normal colon fibroblasts were purchased from American Type Culture Collection (ATCC). LS174T cells were kindly provided by Dr. Ragnhild A. Lothe (Oslo University Hospital, Oslo, Norway). HCT116 cells were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and LS174T and CCD-18Co were cultured in DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). For all cell lines the respective medium was supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂.

All the SCLC cell lines, except DMS53 and DMS114, were obtained from the original NCI-Navy Medical Oncology source supply [44 (link)]. DMS53 and DMS114 SCLC cell lines, CCD-18Co normal colon fibroblasts, and WI-38 lung fibroblasts were purchased from the American Type Culture Collection (ATCC, Manassas, VA). SCLC cell lines were cultured in RPMI-1640 medium (Cat. No. 22400–089, Thermo Fisher, Waltham, MA). CCD-18Co and WI-38 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Cat. No. 12430-062, Thermo Fisher). The culture media were supplemented with 10% heat-inactivated fetal bovine serum (FBS, Cat. No. S11150H, Atlanta Biologicals, GA), 100 U/mL penicillin, and 100 µg/mL streptomycin (Pen-Strep, Cat. No. 15140122, Thermo Fisher). NHBE cells were purchased from Lonza (Cat. No. CC-2541, Basel, Switzerland), and were cultured in Lonza’s bronchial epithelial cell growth medium (Cat. No. CC-3170) with supplements and growth factors (CC-4175). The stocks of NCI SCLC cell lines that we used were STR profiled by NIH, so the authenticity of the cell lines remained preserved. All cultures were confirmed for Mycoplasma negativity using the MycoAlert Mycoplasma Detection Kit (Cat. No. LT07–318). All the cell lines were maintained in a humidified incubator at 37 °C and 5% CO₂.

HCT116 CRC cell line and CCD-18Co normal colon fibroblasts were purchased from the American Type Culture Collection. The LS174T CRC cell line was kindly provided by Dr. Ragnhild A. Lothe (Oslo University Hospital, Oslo, Norway).
HCT116 was cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA); LS174T and CCD-18Co were cultured in DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). For all cell lines, the respective medium was supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). The cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂.