Elastase was measured using the Human PMN-Elastase ELISA Kit (BMS269; Invitrogen) in a neutrophil culture supernatant 6 hours after stimulation. Cell culture supernatant (100 µL) was added to a microwell plate coated with an anti-human polymorphonuclear (PMN) elastase polyclonal antibody and incubated at room temperature for 1 hour with a Horseradish peroxidase-conjugated anti-α 1-proteinase inhibitor antibody. The immune complex was detected by adding tetramethyl-benzidine substrate solution, and the absorbance was determined at 450 nm on a microplate reader (BioTek Synergy H1 Hybrid).
Human pmn elastase elisa kit
Manufactured by Thermo Fisher Scientific
The Human PMN-Elastase ELISA Kit is a quantitative enzyme-linked immunosorbent assay (ELISA) used for the determination of human polymorphonuclear elastase (PMN-Elastase) levels in biological samples. This kit utilizes the sandwich ELISA technique to detect and quantify PMN-Elastase in the sample.
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Lab products found in correlation
2 protocols using human pmn elastase elisa kit
1
Quantifying Neutrophil Elastase Release
2
Maternal Plasma MMP-1 and Elastase Levels
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