Cryospray unit

Manufactured by Bruker

Sourced in Germany

The Cryospray unit is a specialized laboratory equipment designed for sample preparation and preservation. It utilizes cryogenic temperatures to rapidly freeze and preserve samples for further analysis or storage. The Cryospray unit maintains a controlled low-temperature environment to ensure the integrity of the samples during the preparation process.

Automatically generated - may contain errors

Lab products found in correlation

Dataanalysis, by Bruker (2 mentions) Esi tof low concentration tuning mixture, by Agilent Technologies (2 mentions) Maxis plus, by Bruker (1 mentions) Maxis 4g, by Bruker (1 mentions)

3 protocols using cryospray unit

Cryospray-Ionization Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were prepared similarly to the UV-vis spectroscopy section. Cryospray-ionization mass spectrometry measurements were performed on a UHR-TOF Bruker Daltonik maXis Plus, an ESI-quadrupole time-of-flight mass spectrometer capable of a resolution of at least 60.000 (fwhm), which was coupled to a Bruker Daltonik Cryospray unit. Detection was in positive ion mode; the source voltage was 2.5 kV. The flow rate was 240 µL/h. The drying gas (N₂), to achieve solvent removal, was held at –90 °C, and the spray gas was also held at –90 °C. The temperature of the samples injected was also at –90 °C. The mass spectrometer was calibrated prior to every experiment via direct infusion of an Agilent ESI-TOF low concentration tuning mixture, which provided an m/z range of singly charged peaks up to 2700 Da in both ion modes. Simulated spectra were generated in DataAnalysis from Bruker.

Ehudin M.A., Senft L., Franke A., Ivanović-Burmazović I, & Karlin K.D. (2019). Formation and Reactivity of New Isoporphyrins: Implications for Understanding the Tyr-His Cross-Link Cofactor Biogenesis in Cytochrome c Oxidase. Journal of the American Chemical Society, 141(27), 10632-10643.

+ Open protocol

+ Expand

Cryogenic CSI-MS Analysis of Fe-Cu Complex

Check if the same lab product or an alternative is used in the 5 most similar protocols

CSI-MS measurements were performed on a UHR-TOF Bruker Daltonik (Bremen, Germany) maXis 4G, an ESI-TOF MS capable of resolution of at least 40,000 fwhm, which was coupled to a Bruker Daltonik Cryo-spray unit. Detection was in positive-ion mode, and the source voltage was 4.5 kV. The flow rates were 250 µL/hour. The drying gas (N₂), to aid solvent removal, was held at −55 °C, and the spray gas was held at −60 °C.
CSI-MS samples were prepared in a glovebox by dissolving [(TMPP)Fe^III−O−Cu^II(tmpa)][B(C₆F₅)₄] in dry acetone. The complex solution was taken out in a Schlenk tube equipped with a rubber septum. Samples for measurement were taken out directly from this Schlenk tube. The intermediate observed at 433 nm, at −20 °C or below, with two NO molecules added to the µ-oxo complex (thus, a bis-NO adduct) was prepared by bubbling NO_(g) directly through the cooled (−60 °C) complex solution. NO gas was purified as described for the stopped-flow measurements (vide infra).
The machine was calibrated prior to every experiment via direct infusion of the Agilent ESI-TOF low concentration tuning mixture, which provided an m/z range of singly charged peaks up to 2700 Da in both ion modes.

Hematian S., Kenkel I., Shubina T.E., Dürr M., Liu J.J., Siegler M.A., Ivanovic-Burmazovic I, & Karlin K.D. (2015). Nitrogen Oxide Atom-Transfer Redox Chemistry; Mechanism of NO(g) to Nitrite Conversion Utilizing µ-oxo Heme-FeIII−O−CuII(L) Constructs. Journal of the American Chemical Society, 137(20), 6602-6615.

+ Open protocol

+ Expand

Cryospray-Ionization Mass Spectrometry of Superoxide Reactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cryospray-ionization mass spectrometry (CSI-MS) measurements were performed on a UHR-TOF Bruker Daltonik maXis plus, an ESI-quadrupole time-of-flight (qToF) mass spectrometer capable of a resolution of at least 60.000 (FWHM), which was coupled to a Bruker Daltonik Cryospray unit. Detection was in positive ion mode with a source voltage of 3.5 kV and a flow rate of 240 μL per hour. The temperatures of the spray gas (N₂) and the dry gas used for solvent removal were maintained at −40 °C and −35 °C, respectively. The mass spectrometer was calibrated prior to every experiment via direct infusion of an Agilent ESI-TOF low concentration tuning mixture, which provided a m/z range of singly charged peaks up to 2700 Da in both ion modes. For the reactions with O₂˙⁻, 1 mM solutions of either 2 or 3 in MeCN (1% DMF) were cooled to −40 °C and mixed with excess solid KO₂. The mixture was diluted to roughly 1 × 10⁻⁵ M and quickly injected into the mass spectrometer. Applied solvents were not extra dry in order to provide a source of protons. The measured data were processed and analyzed with Bruker Data Analysis. The complete CSI-MS spectra recorded for the reactions of 2 and 3 with excess superoxide are shown in Fig. S1 and S2, respectively, in ESI.†

Senft L., Moore J.L., Franke A., Fisher K.R., Scheitler A., Zahl A., Puchta R., Fehn D., Ison S., Sader S., Ivanović-Burmazović I, & Goldsmith C.R. (2021). Quinol-containing ligands enable high superoxide dismutase activity by modulating coordination number, charge, oxidation states and stability of manganese complexes throughout redox cycling. Chemical Science, 12(31), 10483-10500.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!