Ya0011

Tumor tissues were fixed in 4% formaldehyde, dehydrated with gradient concentrations ethanol, embedded into parrafin (YA0011, Solarbio) and cut into sections with a thick of 5 μm. Sections were retrieved in 10 mM sodium citrate buffer (pH 6.0, P0083, Beyotime) for 15 min at 94°C. Following cooling to room temperature, sections were blocked with 1% bovine serum albumin (BSA, ST2249, Beyotime) for 30 min and then incubated with primary antibodies against RAD54B (1:200, ab238579, Abcam), Ki-67, c-myc (1:1000, ab32072, Abcam) and MMP-7 (1:500, ab216631, Abcam) respectively. Subsequently, sections were incubated with biotinylation-labeled secondary antibody (1:1000, ab207996, Abcam), re-stained with hematoxylin, and captured under a light microscope (Olympus). The relative level of RAD54B, Ki-67, cmyc and MMP-7 was determined as the ratio of the number of positive cells to the total number of cells.

Tumor tissues were fixed in 4% paraformaldehyde, and dehydrated with gradient ethanol. Then, tissues were embedded into paraffin (YA0011, Solarbio) and cut into sections with a thickness of 5 μm. The restoration was executed with sodium citrate buffer (pH 6.0, P0081, Beyotime) at 94 °C for 15 min. Subsequently, sections were sealed with 1% bovine serum albumin (BSA, ST2249, Beyotime) for one hour, and hatched with primary antibodies targeting Ki-67 (1:200, ab15580, Abcam), FOXM1 (1:250, ab207298, Abcam) and STMN1 (1:2000, ab52630, Abcam) overnight at 4 °C. The secondary antibody HRP labeled anti-rabbit IgG antibody (ab288151, Abcam) was used to incubate with sections at 37 °C for 30 min. The sections were re-stained with hematoxylin (G1080, Solarbio), and pictured under a light microscope (Olympus).