Kynurenic acid, MK-801, NaCl, MgCl2, HEPES sodium salt, adenosine triphosphate, guanosine triphosphate, triton-x, and normal goat serum were obtained from Sigma-Aldrich (St. Louis, MO, USA). Neurobiotin was obtained from Vector Laboratories (Peterborough, UK). Streptavidin 488 was obtained from Abcam. K- methylsulfate was obtained from MP Biomedicals (Santa Ana, CA, USA). BAPTA tetrapotassium salt, 10X PBS, and ProLong Gold antifade mountant were obtained from Thermo Fisher Scientific.
Streptavidin 488
Manufactured by Abcam
Sourced in United Kingdom
Streptavidin 488 is a fluorescently labeled streptavidin protein. Streptavidin is a tetrameric protein that binds to the small molecule biotin with high affinity. The Streptavidin 488 conjugate is labeled with the Alexa Fluor 488 fluorescent dye, which has an excitation/emission maxima of 495/519 nm.
Automatically generated - may contain errors
Lab products found in correlation
Kynurenic acid, by Merck Group (1 mentions)
Hepes sodium salt, by Merck Group (1 mentions)
Neurobiotin, by Vector Laboratories (1 mentions)
K methylsulfate, by MP Biomedicals (1 mentions)
Mk 801, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Normal goat serum (ngs), by Merck Group (1 mentions)
Triton x, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Bapta tetrapotassium salt, by Thermo Fisher Scientific (1 mentions)
Guanosine triphosphate, by Merck Group (1 mentions)
Streptavidin hrp, by Bio-Rad (1 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions)
2 protocols using streptavidin 488
1
Immunofluorescence Staining of Neuronal Samples
2
Immunofluorescence Protein Detection Protocol
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The following primary antibodies were used for IF experiments: mouse anti-FLAGm2 (Sigma Aldrich, Oakville, ON, Canada; at 1:500) and rabbit Na/K ATPase (Abcam, Cambridge, UK; at 1:1000). Streptavidin-488 (Abcam, Cambridge, UK; at 1:500) was used to detect biotinylated proteins in IF experiments and Streptavidin-HRP (Bio-Rad, Hercules, CA, USA; 1:5000) was used to detect biotinylated protein in immunoblotting experiments; both without a secondary antibody. Secondary antibodies used for IF were all obtained from Invitrogen, used at 1:1000, and include: Alexa Fluor 488 donkey anti-mouse and Alexa Fluor 647 donkey anti-rabbit (Invitrogen, Burlington, ON, Canada).
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