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D01112603

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D01112603 is a laboratory equipment product. It is a component used in research and scientific applications.

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Lab products found in correlation

D12492, by Research Diets (1 mentions) B6.129s cybbtm1din j, by Jackson ImmunoResearch (1 mentions)

2 protocols using d01112603

1

High-Fat Diet Effects on Mice

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C57BL/6J male mice were housed under a 12-hour light/12-hour dark cycle and allowed free access to food. Mice were fed either a control diet (D12450J, 10kcal% fat, Research Diets, see Table 1), a high lard diet (HLD) (D12492, 60kcal% fat, Research Diets, see Table 1), or a high olive-oil diet (HOD) (D01112603, 60kcal% fat, Research Diets, see Table 1). Mice were fed with either an HLD or HOD starting at 4 weeks of age or kept on a control diet. We analyzed mice at 20 weeks of age. Euthanasia was performed by Pentobarbital intraperitoneal overdose (300mg/kg).
Yamamoto T., Endo J., Kataoka M., Matsuhashi T., Katsumata Y., Shirakawa K., Yoshida N., Isobe S., Moriyama H., Goto S., Yamashita K., Nakanishi H., Shimanaka Y., Kono N., Shinmura K., Arai H., Fukuda K, & Sano M. (2018). Decrease in membrane phospholipids unsaturation correlates with myocardial diastolic dysfunction. PLoS ONE, 13(12), e0208396.
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2

High-fat Diet Mouse Cardiomyocyte Isolation

Cited 1 time
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Animal protocols were approved by the Columbia University Institutional Animal Care and Use Committee and were carried out in accordance with the NIH guidelines for the care and use of laboratory animals. Wild type (WT) and B6.129S-Cybbtm1Din/J (NOX2KO) mice were purchased from Jackson labs and were used for experiments at 12–16 weeks of age. High fat diets were special orders from Research Diets Inc: D04051707, 60 kcal% from palm oil, 20% kcal protein, 20% kcal carbohydrate; and D01112603, 60 kcal% from olive oil, 20% kcal protein, 20% kcal carbohydrate. Control chow was Picolab Rodent diet 20 from LabDiet, which has 13% kcal from fat, 25% protein, and 62% carbohydrate. Animals were fed ad libitum. Isolation of cardiomyocytes was performed as previously described6 (link). Animals were randomly assigned to treatment groups. No animals were excluded from the analysis. Echocardiograms, optical mapping, and histology quantification were performed by blinded operators.
Joseph L.C., Avula U.M., Wan E.Y., Reyes M.V., Lakkadi K.R., Subramanyam P., Nakanishi K., Homma S., Muchir A., Pajvani U.B., Thorp E.B., Reiken S.R., Marks A.R., Colecraft H.M, & Morrow J.P. (2019). Dietary Saturated Fat Promotes Arrhythmia by Activating NADPH Oxidase 2 (NOX2). Circulation. Arrhythmia and electrophysiology, 12(11), e007573.
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