Anti mcherry

Animals were transcardially perfused with PBS followed by 4% paraformaldehyde. Brains were dissected and post-fixed for 16–24 h at 4 °C. Brains were sectioned using a vibratome (Leica) to a thickness of 50 µm. Samples were then stained with the following primary antibodies: anti-GFP (Aves Labs GFP-1020) at 1:1,000, anti-mCherry (Rockland 600-401-379) at 1:1,000 or anti-FOS antibody (Synaptic Systems 226 003) at 1:2,000 in PTxwH buffer for 24 h at 4 °C. After four 15-min washes in PTxwH buffer, samples were incubated in Alexa Fluor-conjugated secondary antibodies (Thermo Fisher) at 1:2,000 for 90 min at room temperature, followed by four further 15-min washes before mounting using 4′,6-diamidino-2-phenylindole Fluoromount-G (SouthernBiotech 0100-20 OB010020).

The following primary antibodies were used for immunostaining and/or immunoblotting: mouse anti-F (BioRad, Hercules, CA, USA), mouse anti-F 2F7 (Abcam, Cambridge, UK), mouse anti-G (Abcam), rabbit anti-N antiserum [54 (link)], rabbit anti-GM130 (Abcam), mouse anti-Syntaxin-5 (Santa Cruz, Dallas, TX, USA), mouse monoclonal anti-β tubulin antibody (Sigma-Aldrich, Saint-Louis, MO, USA), rabbit anti-GFP (Invitrogen, Whaltham, MA, USA), and anti-mCherry (Rockland immunochemicals, Limerick, ME, USA).
Secondary antibodies directed against mouse and rabbit IgG coupled to HRP (SeraCare, Milford, CT, USA) were used for immunoblotting, and antibodies directed against mouse and rabbit IgG coupled to Alexa 594 or Alexa 488 (Invitrogen), respectively, were used for immunostaining.