Log In Sign up
The largest database of trusted experimental protocols

Live dead fixable dye staining kit

Manufactured by BioLegend
Visit Supplier

The Live/Dead Fixable Dye Staining Kit is a laboratory reagent used to distinguish between live and dead cells in a sample. It provides a simple and effective method for detecting cell viability during various experimental procedures, such as flow cytometry. The kit includes a fixable dye that labels dead cells, allowing for their identification and separation from the live cell population.

Automatically generated - may contain errors

Lab products found in correlation

Canto 2, by BD (2 mentions) Anti ifn γ bv421, by BD (1 mentions) Anti granzyme b af647, by BioLegend (1 mentions) Cytofix perm buffer, by BD (1 mentions) Perm buffer 3, by BD (1 mentions)

2 protocols using live dead fixable dye staining kit

1

Multiparameter analysis of immune cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dead cells were excluded using a live/ dead fixable dye staining kit (BioLegend). Cells were stained for surface markers and then fixed and permeabilized with CytoFix/Perm Buffer (BD). Intracellular molecules were detected using anti-IFN-γ-BV421 (BD) and anti-caspase 3-AF647 (BD) and anti-Granzyme B AF647 (BioLegend). For intranuclear staining, the True nuclear transcription buffer set from Biolegend was used according to the manufacturer protocol. Antibody against anti-Ki67 AF488 (BioLegend) was used. All samples were acquired on a BD Canto II. All analyses were performed using Flowjo software (Tree Star).
Otano I., Alvarez M., Minute L., Ochoa M.C., Migueliz I., Molina C., Azpilikueta A., de Andrea C.E., Etxeberria I., Sanmamed M.F., Teijeira Á., Berraondo P, & Melero I. (2020). Human CD8 T cells are susceptible to TNF-mediated activation-induced cell death. Theranostics, 10(10), 4481-4489.
+ Open protocol
+ Expand
2

Analysis of DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dead cells were excluded using a live/dead fixable dye staining kit (BioLegend). Cells were stained for surface markers and then fixed with 4% of paraformaldehyde. Cells were then permeabilized with cold Perm III Buffer (BD). Then, gH2AX-PE (BioLegend) was detected by intranuclear staining. All samples were acquired on a BD Canto II. All analyses were performed using Flowjo software (Tree Star).
Otano I., Alvarez M., Minute L., Ochoa M.C., Migueliz I., Molina C., Azpilikueta A., de Andrea C.E., Etxeberria I., Sanmamed M.F., Teijeira Á., Berraondo P, & Melero I. (2020). Human CD8 T cells are susceptible to TNF-mediated activation-induced cell death. Theranostics, 10(10), 4481-4489.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!