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Cy3 fluorescent dye

Manufactured by Merck Group
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Cy3 is a fluorescent dye commonly used in molecular biology applications. It has an excitation wavelength of approximately 550 nm and an emission wavelength of approximately 570 nm. Cy3 can be used to label biomolecules such as proteins, nucleic acids, and antibodies for detection and visualization purposes.

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Lab products found in correlation

Confocal laser scanning microscope, by Olympus (1 mentions) Fluorescent in situ hybridization kit, by RiboBio (1 mentions) Bca assay kit, by Merck Group (1 mentions) Sephadex g 25 column, by Merck Group (1 mentions)

Close spelling variants of this product

Cy3 dye Fluorescent dyes

2 protocols using cy3 fluorescent dye

1

Visualizing Circular RNA Expression

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Using fluorescent in situ hybridization kit (RiboBio), RNA FISH were carried out. The circ-GLI1 probes were labeled with Cy3 fluorescent dye (Sigma-Aldrich). Fluorescence signals were detected by confocal laser-scanning microscope (Olympus, Tokyo, Japan). All samples were assayed in triplicate.
Chen J., Zhou X., Yang J., Sun Q., Liu Y., Li N., Zhang Z, & Xu H. (2020). Circ-GLI1 promotes metastasis in melanoma through interacting with p70S6K2 to activate Hedgehog/GLI1 and Wnt/β-catenin pathways and upregulate Cyr61. Cell Death & Disease, 11(7), 596.
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2

Mitochondrial Protein Quantification and Labeling

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To allow for individual variations and to normalize the differences, five mitochondrial protein samples from each group (SAD-C, SAD, MC, M16 or M48) were pooled and quantified using a BCA assay kit (Cat No.: QPBCA, Sigma) respectively. Proteins were labeled with Cy3 fluorescent dye (Sigma) and purified with Sephadex G-25 columns (Cat No.: G2580, Sigma) as per the product specifications.
Yu H., Yang C., Chen S., Huang Y., Liu C., Liu J, & Yin W. (2017). Comparison of the glycopattern alterations of mitochondrial proteins in cerebral cortex between rat Alzheimer’s disease and the cerebral ischemia model. Scientific Reports, 7, 39948.
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