Percoll
Percoll is a colloidal silica-based medium used for cell separation and gradient centrifugation. It is designed to provide a density gradient for the isolation and purification of cells, organelles, and other biological particles.
Lab products found in correlation
1 443 protocols using percoll
Percoll Density Gradient Fractionation
RBC Separation on Percoll Gradient
Isolation and Characterization of Mouse and Human Liver Sinusoidal Endothelial Cells
Isolation of Human and Murine Neutrophils
Isolation of Lamina Propria Cells
Isolating Colonic Lamina Propria Cells
Isolation of Intestinal Lymphocytes
Isolation of Human Blood Leukocytes
et al. (2015)
23 (link)
. Briefly, blood was first centrifuged at 300 × g (acceleration 5, deceleration 5) for 20 minutes and the platelet-rich plasma layer removed. Erythrocyte sedimentation and leukocyte-rich plasma were obtained by incubating the remaining contents in the tube with 6 mL of 6% Dextran 500 (Pharmacosmos) in saline and final volume adjusted to 50 mL with 0.9% NaCl (Baxter) for at least 20 minutes at room temperature. The leukocyte-rich portion was centrifuged at 350 × g (acceleration 5, deceleration 5) for 6 minutes, with the pellet resuspended in 3 mL of 49.5% Percoll (GE Healthcare) and overlayed onto 61.2% Percoll and 72.9% Percoll. Gradients were centrifuged at 720 × g (acceleration 1, deceleration 0) for 20 minutes to obtain PMN and PBMC layers.
Isolation of Liver Mononuclear Cells
Isolation of Mouse Hematopoietic Cells
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