Gcms qp 2010 chromatograph

A Shimadzu GCMS-QP 2010 chromatograph (Kyoto, Japan) with a DB-5 capillary column (30 m × 0.25 mm i.d. × 0.25 μm film thickness; Restek, Bellefonte, PA, USA) was used for gas chromatography/mass spectrometry (GC/MS) analysis. The oven temperature was set at 50 °C for 3 min (isothermal), programmed to 30 °C at a rate of 5 °C/min, and kept constant at 300 °C for 10 min (isothermal); the temperature of the injector was 280 °C. Helium was employed as the carrier gas, with a flow rate of 1.40 mL/min. Diluted samples (1% v/v) were injected at a split ratio of 15:1 in a volume of 1 μL. The following were the MS running specifications: 280 °C for the interface, 220 °C for the ion source, 70 eV for the EI mode, and 35–500 amu for the scan range.

Gas chromatography/Mass spectrometry (GC/MS) analysis was carried out on a Shimadzu GCMS-QP 2010 chromatograph (Kyoto, Japan) with Rtx-1MS capillary column (30 m × 0.25 mm i.d. × 0.25 μm film thickness; Restek, Bellefonte, PA, USA). The oven temperature was kept at 45 °C for 2 min (isothermal), programmed to 30 °C at a rate of 5 °C/min, and kept constant at 300 °C for 5 min (isothermal); injector temperature was 250 °C. The carrier gas used was helium, with a flow rate set at 1.40 mL/min. The diluted samples (1% v/v) were injected with a split ratio of 15:1 and the injected volume was 1 μL. The MS operating parameters were as follows: interface temperature 280 °C, ion-source temperature 220 °C, EI mode 70 eV, scan range 35–500 amu. Identification of the volatile constituents was made based on their retention indices, matching their fragmentation patterns with the NIST Mass Spectral Library, the Wiley library database, and the published data in the literature [78 ,79 ,80 (link),81 (link)]. Retention indices (RI) were calculated relative to the homologous series of n-alkanes (C8–C30) and injected under the same conditions.