C57bl 6j wt

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The C57BL/6J (WT) is a laboratory mouse strain commonly used in biomedical research. It is a wild-type (WT) strain, which means it does not have any known genetic modifications. This mouse strain is widely utilized as a control or reference strain in various experimental studies.

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153 protocols using c57bl 6j wt

Evaluating Mouse Genetic Strains

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All experiments were carried out in male and female mice. The following mouse strains were used: ICR outbred mice purchased from Envigo Laboratories (4–6 weeks, weighing from 20 to 25 g, RRID: IMSR_CRL:022); Mknk1^−/− (MNK1 KO), a gift from the Sonenberg laboratory at McGill University and their C57BL/6J WT (WT) littermates (26 (link), 27 (link)) bred at the University of Texas at Dallas (UTD); Sting^Gt/Gt also known as Goldenticket mice (Tmem173^Gt) and the suggested controls C57BL/6J WT (Lot: 000664) purchased from Jackson laboratory.
Purchased mice were used in experiments starting one week after arrival at the animal facilities at the University of Texas at Dallas. Animals had free access to food and water before the experiments. Mice were housed in groups of 4 per cage in non-environmentally enriched cages with food and water ad libitum on a 12 h non-inverted light/dark cycle. All animal procedures were approved by The University of Texas at Dallas IACUC. Experiments were in compliance with the National Institutes of Health Guide for Care and Use of Laboratory Animals (Publication No. 85-23, revised 1985). Animals were monitored for health according to IACUC guidelines at The University of Texas at Dallas prior and during experimentation. This study was not pre-registered.

Franco-Enzástiga Ú., Natarajan K., David E.T., Patel K.J., Ravirala A, & Price T.J. (2023). Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction. bioRxiv.

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Mouse Models for MNK1 and STING Studies

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All experiments were carried out in male and female mice. The following mouse strains were used: ICR outbred mice purchased from Envigo Laboratories (4–6 weeks, weighing from 20 to 25 g, RRID: IMSR_CRL:022); Mknk1^−/− (MNK1 KO), a gift from the Sonenberg laboratory at McGill University and their C57BL/6J WT (WT) littermates⁴³ (link)^,⁸² (link) bred at the University of Texas at Dallas (UTD); Sting^Gt/Gt also known as Goldenticket mice (Tmem173Gt) and the suggested controls C57BL/6J WT (Lot: 000664) purchased from Jackson laboratory.
Purchased mice were used in experiments starting one week after arrival at the animal facilities at the University of Texas at Dallas. Animals had free access to food and water before the experiments. Mice were housed in groups of 4 per cage in non-environmentally enriched cages with food and water ad libitum on a 12 h non-inverted light/dark cycle. All animal procedures were approved by The University of Texas at Dallas IACUC. Experiments were in compliance with the National Institutes of Health Guide for Care and Use of Laboratory Animals (Publication No. 85-23, revised 1985). Animals were monitored for health according to IACUC guidelines at The University of Texas at Dallas prior and during experimentation. This study was not pre-registered.

Franco-Enzástiga Ú., Natarajan K., David E.T., Patel K., Ravirala A, & Price T.J. (2024). Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction. iScience, 27(2), 108808.

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Genetic Mouse Models for Colorectal Cancer

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WT C57BL/6J (Cat # 000664), APC^min/+ (Cat # 002020), Lgr5-EGFP-IRES-creERT2 (Cat # 008875) and APC^flox mice (Cat # 009045) were purchased from Jackson Laboratory. FXR^flox mice were kindly provided by Dr. Pierre Chambon (University of Strasbourg, France) and maintained in the Evan’s laboratory. All animal experiments were performed in the specific pathogen-free facilities at the Salk Institute following the Institutional Animal Care and Use Committee’s guidelines.

Fu T., Coulter S., Yoshihara E., Oh T.G., Fang S., Cayabyab F., Zhu Q., Zhang T., Leblanc M., Liu S., He M., Waizenegger W., Gasser E., Schnabl B., Atkins A.R., Yu R.T., Knight R., Liddle C., Downes M, & Evans R.M. (2019). FXR regulates intestinal cancer stem cell proliferation. Cell, 176(5), 1098-1112.e18.

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Genetic Manipulation for Inflammasome Study

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Aim2^−/−, Nlrp3^−/−, Il18^−/−, Caspase-1^−/− (Caspase-1/11^−/−) and WT (C57Bl6/J) mice were purchased from Jackson Laboratory. Il1β^−/− mice (Shornick et al., 1996 (link)) were kindly shared by Dr. Chandrashekhar Pasare at UT Southwestern. All mice are maintained in a specific pathogen free (SPF) facility. All studies were approved by the Institutional Animal Care and Use Committee (IACUC) of UT Southwestern Medical Center and were conducted in accordance with the IACUC guidelines and the National Institutes of Health Guide for the Care and Use of Laboratory Animals. All experiments were conducted with sex and age-matched mice and both male and female mice were included.

Hu S., Peng L., Kwak Y.T., Tekippe E.M., Pasare C., Malter J.S., Hooper L.V, & Zaki M.H. (2015). The DNA Sensor AIM2 Maintains Intestinal Homeostasis via Regulation of Epithelial Antimicrobial Host Defense. Cell reports, 13(9), 1922-1936.

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AhR-Deficient Mice and Bone Marrow Chimeras

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AhR^−/− mice (C57BL/6J; generation 19; Schmidt et al., 1996 (link)) bred as heterozygous and littermates (6–10 wk) were used for experiments, or AhR^−/− males were bred to AhR^+/− females, and age/sex-matched WT C57BL6/J (purchased from The Jackson Laboratory) mice were used as controls. For BM chimeras, WT CD45.1 congenic and AhR^−/− mice (or WT CD45.2 C57BL6/J; 6–8-wk-old females) were used as a source of BM cells. Single suspensions of BM extracted from femurs and tibias were depleted of T cells with Thy1 antibody–coated magnetic beads. 3–5 × 10⁶ T cell–depleted BM cells were transferred (intravenous) individually or at a 1:1 ratio into irradiated (650 cGy) 8–10-wk-old Rag1^−/− female recipients (The Jackson Laboratory). BM engraftment was examined by flow cytometric analysis of peripheral blood lymphocytes 8–10 wk after BM transfer. Three independent sets of chimera were generated. Mice were cheek bled at 7–8 wk after reconstitution to determine chimerism of B, T, and myeloid cells by flow cytometry. All animals were maintained and euthanized as per the Memorial Sloan Kettering Cancer Center Research and animal resource center guidelines.

Vaidyanathan B., Chaudhry A., Yewdell W.T., Angeletti D., Yen W.F., Wheatley A.K., Bradfield C.A., McDermott A.B., Yewdell J.W., Rudensky A.Y, & Chaudhuri J. (2017). The aryl hydrocarbon receptor controls cell-fate decisions in B cells. The Journal of Experimental Medicine, 214(1), 197-208.

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Studying Pde6rd10 Mouse Model

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B6.CXB1-Pde6^rd10/J (rd10) and WT C57BL/6J mice were purchased from The Jackson Laboratories (Bar Harbor, ME, USA).

Murakami Y., Ikeda Y., Nakatake S., Tachibana T., Fujiwara K., Yoshida N., Notomi S., Nakao S., Hisatomi T., Miller J.W., Vavvas D., Sonoda K, & Ishibashi T. (2015). Necrotic enlargement of cone photoreceptor cells and the release of high-mobility group box-1 in retinitis pigmentosa. Cell Death Discovery, 1, 15058-.

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Gene-Edited Mice for Immunology Research

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WT C57BL/6J (stock no. 000664) mice were purchased from Jackson Laboratories and maintained at Washington University School of Medicine under specific-pathogen-free conditions according to University guidelines. Rag2^−/−Il2rg^−/− mice were generated at Washington University School of Medicine by crossing of Il2rg^−/− (B6.129S4-Il2rg^tm1Wjl/J, catalogue no. 003174) mice from Jackson Laboratories with Rag2^−/− (B6.129S6-Rag2^tm1FwaN12, catalogue no. RAGN12) mice from Taconic. Rag2^−/−Il2rg^−/−Ifnlr1^−/− mice were generated as previously described^{9 (link)}. Rag2^−/−Il2rg^−/− mice from Taconic were used directly for experiments on arrival at Washington University facilities. Math1^f/f mice were crossed to transgenic mice bearing a tamoxifen-dependent Cre recombinase expressed under the control of the Villin promoter (Vil-Cre-ERT2)^{30 (link)} to generate Math1^f/f-Vil-Cre-ERT2 mice as previously described^{21 (link)}. Germ-free Swiss Webster mice were purchased from Taconic Biosciences and maintained in sterile, flexible-film plastic gnotobiotic isolators at the Washington University School of Medicine Gnotobiotic Facility. Equal ratios of adult male and female mice, aged 6 to 12 weeks, were used in all experiments for all strains. Experimental mice were co-housed with up to five mice of the same sex per cage with autoclaved standard chow pellets and water provided ad libitum.

Ingle H., Hassan E., Gawron J., Mihi B., Li Y., Kennedy E.A., Kalugotla G., Makimaa H., Lee S., Desai P., McDonald K.G., Diamond M.S., Newberry R.D., Good M, & Baldridge M.T. (2021). Murine astrovirus tropism for goblet cells and enterocytes facilitates an IFN-λ response in vivo and in enteroid cultures. Mucosal immunology, 14(3), 751-761.

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Liver-Specific PPAR-γ Knockout Mice

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Liver-specific PPARγ knockout (KO) mice were generated by breeding PPARγ-loxP mice (The Jackson Laboratory, Bar Harbor, ME) to transgenic Alb-Cre (B6.Cg-Tg (Alb-Cre) 21Mgn/J, The Jackson Laboratory) mice as previously described [25 (link)]. Two-month-old male KO or control (i.e., wildtype (WT) C57BL6/J; The Jackson Laboratory) mice were given ad lib access to sterile, irradiated low-fat (S4031, BioServ, Flemington, NJ) or high-fat (S3282, BioServ; 60% kcal from fat) diets, with or without 0.01% (w/w) pioglitazone hydrochloride (Pio, Sigma-Aldrich, St. Louis, MO) for 3 months (n = 4 − 5 mice/experimental group). Body weight was measured weekly and body composition analyzed at the experimental endpoint by ECHO MRI spectroscopy [26 (link)]; after which, mice were euthanized for tissue harvest and analysis. Blood glucose, serum insulin and free fatty acid levels, and liver histology and triglyceride content were determined as previously described [25 (link)–27 (link)]. All experiments were approved by the Washington University Institutional Animal Care and Use Committee (IACUC), and all animals received humane care in accordance with institutional guidelines and criteria in the “Guide for the Care and Use of Laboratory Animals” (8^th edition, 2011, https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory-animals.pdf).

Kulkarni S., Huang J., Tycksen E., Cliften P.F, & Rudnick D.A. (2020). Diet Modifies Pioglitazone's Influence on Hepatic PPARγ-Regulated Mitochondrial Gene Expression. PPAR Research, 2020, 3817573.

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Gene-Edited Mice for Immunology Research

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Hyperhomocysteinemia Induction in Mice

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Male and female 10–12 weeks old mice, WT (C57BL/6J) and CBS^+/− (B6.129P2-Cbstm1Unc/J 002853) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). The animal procedures were reviewed and subsequently approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Louisville School of Medicine, Louisville, Kentucky, USA. Further, the animal care and guidelines of the National Institutes of Health (NIH, USA) were also adhered to. To create HHcy condition, the mice were fed with a high methionine diet HMD (Harlan Laboratories) and with or without oral probiotic; PB (Lactobacillus rhamnosus GG @ 2.5×10⁵ CFU for a period of 16 weeks in drinking water. Control mice groups were fed the standard chow diet. All mice were allowed water ad libitum.

George A.K., Singh M., Pushpakumar S., Homme R.P., Hardin S.J, & Tyagi S.C. (2019). Dysbiotic 1-carbon metabolism in cardiac muscle remodeling. Journal of cellular physiology, 235(3), 2590-2598.

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