The U87, U118, and Hs5 cell lines (5 × 104 cells per well) were seed onto 6-well plates and incubated overnight. Then, they were treated with NDs at concentrations of 5 and 50 μg/mL. Cells cultured without the addition of nanoparticles were used as the control group. After 24 and 72 h of incubation, the changes in the morphology of the cells was investigated using a CKX 41 epifluorescent inverted microscope with a fluorescent filter (Olympus, Tokyo, Japan). The images were captured using a ProgRes® c12 camera (Jenoptik, Jena, Germany).
Progres c12 camera
Manufactured by Jenoptik
Sourced in Germany
The ProgRes® c12 is a digital camera designed for microscopy applications. It features a high-resolution 12-megapixel CMOS sensor and supports various image formats, including TIFF, JPEG, and BMP. The camera is capable of capturing images with a maximum resolution of 4,000 x 3,000 pixels and offers a wide range of adjustable parameters, such as exposure time, gain, and white balance.
Automatically generated - may contain errors
4 protocols using progres c12 camera
1
Nanoparticle Effects on Cell Morphology
2
Morphological Analysis of Nanoparticle Cytotoxicity
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After 24 h of exposure to the nanoparticles or cisplatin, the medium was removed and the cells were stained using the May-Grünwald-Giemsa (Sigma-Aldrich) method, and their morphology was investigated using a CKX 41 light microscope (Olympus, Tokyo, Japan). Images were captured using a ProgRes c12 camera (Jenoptik, Jena, Germany).
3
Morphological Analysis of MEL-Nanoparticle Effects
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After 24 h of exposure to the MEL and MEL–nanoparticles complexes, the medium was removed, the cells were stained using the May–Grünwald–Giemsa (Sigma-Aldrich) method, and their morphology was investigated using a CKX 41 light microscope (Olympus, Tokyo, Japan). Images were captured using a ProgRes c12 camera (Jenoptik, Jena, Germany). Cells cultured without the addition of MEL–nanoparticles or MEL, but with the same volume of media, were used as the control group.
4
Nanoparticle Effects on U87 Cell Morphology
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The U87 cells were incubated on six-well plates (1×105 cells per well) and cultivated for 24 hours. GN, rGO, GO, graphite, and UDD were introduced to the cells separately at a constant concentration of 50 μg/mL. Cells cultured without the addition of nanoparticles were used as the control group. After 24 hours of exposure to the nanoparticles, the medium was removed and the cells were stained using the May-Grünwald-Giemsa (Sigma-Aldrich) method, and their morphology was investigated using a CKX 41 epifluorescent inverted microscope with a fluorescent filter (Olympus, Tokyo, Japan); images were captured using a ProgRes® c12 camera (Jenoptik, Jena, Germany).
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