The rats were anesthetized on the twenty-fifth day of this experiment and then extracted 5.0 mL aortic blood from the abdomen. The blood was placed in the centrifuge tube for 2 hours, and centrifuged with 3000 rpm at 4°C for 15 minutes. The serum was separated into 1.5 mL Eppendorf tubes and stored in a refrigerator at -20°C. The levels of interleukin (IL)-2, interferon gamma (INF-γ), and tumor necrosis factor alpha (TNF-α) in serum of rats were determined by ELISA kits (IL-2: ab223588, Abcam, UK; INF-γ: 1009251, Shanghai Westang Bio-Tech Co., Ltd., China; TNF-α: ab208348, Abcam, UK) according to the kit instruction.
Ab223588
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab223588 is a lab equipment product offered by Abcam. It is a device designed for use in laboratory settings, but no further details about its core function can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab208348, by Abcam (1 mentions)
Varioskan, by Thermo Fisher Scientific (1 mentions)
Ab263882, by Abcam (1 mentions)
Ab34139, by Abcam (1 mentions)
Ab100747, by Abcam (1 mentions)
Ab234579, by Abcam (1 mentions)
Ab100712, by Abcam (1 mentions)
Ab33471, by Abcam (1 mentions)
Ab37068, by Abcam (1 mentions)
Ab100705, by Abcam (1 mentions)
Ab199081, by Abcam (1 mentions)
3 protocols using ab223588
1
Serum Cytokine Analysis in Rats
2
Measurement of Serum IL-2 Levels
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Twenty-four hours after the ultrasound treatment, the tumor-bearing mice were sacrificed, and 100 µl of blood was collected from the heart. After clotting and centrifuging, the serum was obtained. IL-2 levels of the serum were measured by a sandwich ELISA using a reagent kit (ab223588, Abcam, USA) according to the manufacturer’s instructions. The difference between the color of the analyte and standard is measured by a microplate reader (Thermo Scientific Varioskan™, USA), and the enzyme activity curve is drawn to calculate the concentration of the analyte.
3
Serum Biomarker Profiling in Rats
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On week 4, blood samples (1 ml) were collected through cardiac puncture during sacrifice and serum was separated using centrifugation at 10,000 × g for 5 min at 4°C. Serum levels of TNF-α (ab100747, Abcam), IL-1β (ab100705, Abcam), IL-17A (ab199081, Abcam), IL-6 (ab100712, Abcam), TGF-β1 (ab118557, Abcam), IL-2 (ab223588, Abcam), IL-10 (ab33471, Abcam), ALT (ab234579, Abcam), AST (ab263882, Abcam), γ-GT (ab134640, Abcam), ALP (ab256583, Abcam), DBIL (ab34139, Abcam) and TBIL (ab37068, Abcam) were measured by using rat-specific sandwich ELISA (Abcam) according to the manufacturer's protocol.
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