This test took place in a fear-conditioning apparatus (StartFear, Panlab Harvard Apparatus, 25 cm height × 30 cm width × 25 cm depth) on two consecutive days as described.18 (link) During the training phase (Day 1), animals were placed in the chamber, and baseline freezing was recorded during a 6-min time interval with three cycles of 30s of sound and 10 s of electric shock (1.5mA). During the memory test phase (Day 2), mice were assessed for two trials, contextual and cued memory. For the contextual test, mice spent 5 min in the same chamber used during training, but without tones or electric shocks, and freezing behavior was recorded. For the cued test, two hours following the contextual test mice were placed in the same chamber with a modified environment such as different walls, smells, lighting, flooring, sound and freezing behavior was recorded for 6 min. Differences in freezing time between groups were analyzed via PACKWIN (Panlab, Harvard Apparatus, USA).
Packwin
Manufactured by Harvard Apparatus
Sourced in United States, Spain
Packwin is a software package designed for data acquisition and analysis. It provides a user-friendly interface for controlling and monitoring various laboratory instruments and experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using packwin
1
Fear Conditioning Behavioral Assay
2
Fear Conditioning and Contextual Testing in Mice
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The fear conditioning test was performed as described with modification [23 (link)]. Mice were placed into a shock chamber and allowed to explore for 2 min. Then, a white noise tone (87 dB) sounded for 30 s (conditional stimulus or “CS”). During the last 1.5 s of the tone, mice received a mild footshock (0.5 mA) (unconditioned stimulus or “US”). 2 min later, the same tone-footshock (CS-US) combination was delivered again. This cycle was presented a total of three times with a 60-s interval. The context test was performed 24 h after the training. During the test, mice were placed back into the same training chamber, and monitored by an overhead camera in the chamber for 5 min. No stimuli were applied. Two-hours after the context test, the cue test was performed, in which colored plexiglass inserts were placed into the training chamber to hide the shock grid and to change the “context” of the chamber. Mice were then placed in the chamber and monitored by the overhead camera for 6 min, during which two CS (spaced the same way as in the training session) were given. In the fear conditioning test, all events were programmed and all data were recorded through the Startle and Fear conditioning system (Panlab) and Packwin software (V2.0.05).
3
Contextual Fear Conditioning in Mice
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The fear conditioning test was performed as described32 (link). Mice were habituated to a shock chamber for 2 min and then received a mild footshock (0.5 mA) (unconditioned stimulus or “US”) during the last 1.5 s of a 30-s white noise tone (87 dB) (conditional stimulus or “CS”). Two minutes later, mice received the same tone-footshock pairing (CS–US). The cycle was performed three times. After 24 h, the context test was performed by placing the mice back into the same chamber and monitoring them for 5 min. The cue test was performed 2 h later, in which colored plexiglass inserts were used to change the chamber surrounding. Mice were placed in and monitored for 6 min with two CS (administered in the same way as in the training session) given in the meantime. All events were programmed in the fear conditioning test, and the data were recorded using the Startle and Fear conditioning system (Panlab) and Packwin software (V2.0.05).
4
Contextual Fear Conditioning in Mice
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The contextual fear conditioning test was performed using a method similar to a previous report10 (link) with minor modifications. Mice were handled 10 min per day for 3 days before the tests. Fear conditioning was conducted in a chamber (25 × 25 × 25 cm; LE116, Panlab, Spain) with a stainless-steel grid floor. Each mouse was placed in the chamber and was allowed to explore freely for 5 min per day for 3 days to acclimate to the chamber. Twenty-four hours after the acclimation, a 30-s sound of 85 dB was presented through a speaker set on top of the conditioning chamber, which served as the conditioning stimulus (CS). During the last 2 s of the CS presentation, mice received a footshock (0.75 mA, 2 s), which served as an unconditioned stimulus (US). Animals were returned to their home cages 1.5 min after the CS-US pairing. Twenty-four hours after the conditioning, contextual fear memory was tested for 4 min in the same chamber. The percentage of time of freezing of the animal was provided by the software (Packwin, panlab, Spain).
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