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Anti lin28b antibody

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The Anti-LIN28B antibody is a laboratory tool used to detect and study the expression of the LIN28B protein in various biological samples. LIN28B is a regulator of cell growth and development. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate the presence and levels of LIN28B in cells and tissues.

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Lab products found in correlation

Blue loading buffer pack, by Cell Signaling Technology (2 mentions) Novex 16 tris glycine mini gels, by Thermo Fisher Scientific (2 mentions) Anti actb mouse monoclonal antibody, by GenScript (2 mentions) Thermo scientific myecl imager, by Thermo Fisher Scientific (2 mentions) Anti gapdh, by Thermo Fisher Scientific (1 mentions) Anti oct4 antibody, by Santa Cruz Biotechnology (1 mentions) Hybond nitrocellulose membrane, by Cytiva (1 mentions)

5 protocols using anti lin28b antibody

1

LIN28B Western Blot Protocol

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Whole-cell lysates for Western blots were extracted with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S). Protein lysates were loaded into Novex 16% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP99165BOX) and separated by gel electrophoresis at 125V for 4 hours. LIN28B and β-actin were detected using an anti-LIN28B antibody (Cell Signaling Technology, #4196) and an anti-ACTB mouse monoclonal antibody (GenScript, A00702), respectively. More details about the antibodies can be found in the Life Sciences Reporting Summary. The Western blot was imaged with Thermo Scientific myECL Imager (Thermo Scientific, 62236).
Jang H.S., Shah N.M., Du A.Y., Dailey Z.Z., Pehrsson E.C., Godoy P.M., Zhang D., Li D., Xing X., Kim S., O’Donnell D., Gordon J.I, & Wang T. (2019). Transposable elements drive widespread expression of oncogenes in human cancers. Nature genetics, 51(4), 611-617.
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2

Immunohistochemical Analysis of LIN28B in Pediatric Tumors

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Performed on human tumor tissue sections as previously described4 (link) using anti-LIN28B antibody (Cell Signaling #4196) at a 1:400 dilution. Patient samples were obtained through Boston Children’s Hospital IRB-CRS08-09-0429-2; Immunohistochemical and Molecular Analysis of Pediatric Tumors and consent was obtained from all subjects.
Powers J.T., Tsanov K.M., Pearson D.S., Roels F., Spina C.S., Ebright R., Seligson M., de Soysa Y., Cahan P., Theiβen J., Tu H.C., Han A., Kurek K.C., LaPier G.S., Osborne J.K., Ross S.J., Cesana M., Collins J.J., Berthold F, & Daley G.Q. (2016). Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma. Nature, 535(7611), 246-251.
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3

Immunoblotting for Stem Cell Markers

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Cells were lysed and sonicated in RIPA buffer, following SDS-PAGE of lysates. Proteins were transferred to Hybond nitrocellulose membranes (Amersham, GE Healthcare) and immunoblotted with anti-OCT4 antibody (1∶1,000; sc-5279; Santa Cruz Biotechnology), anti-LIN28B antibody (1∶1,000; #4196, Cell signalling) and anti-GAPDH (1∶10,000; #4300, Ambion). After washing with PBST, secondary HRP-linked antibody ECL Mouse IgG, (1∶5,000; Amersham) were used and detected with enhanced ECL reagent (Amersham). Figure 4D, Chemiluminescent Detection Films (Roche) and CURIX 60 film developing machine (Agfa) was used to visualize ECL-activity. Figure 3B, ECL-activity was detected with a FUSION-FX7 Advance Chemiluminescent system (Peqlab).
Fuchs H., Theuser M., Wruck W, & Adjaye J. (2014). miR-27 Negatively Regulates Pluripotency-Associated Genes in Human Embryonal Carcinoma Cells. PLoS ONE, 9(11), e111637.
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4

Immunohistochemical Analysis of LIN28B in Pediatric Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Performed on human tumor tissue sections as previously described4 (link) using anti-LIN28B antibody (Cell Signaling #4196) at a 1:400 dilution. Patient samples were obtained through Boston Children’s Hospital IRB-CRS08-09-0429-2; Immunohistochemical and Molecular Analysis of Pediatric Tumors and consent was obtained from all subjects.
Powers J.T., Tsanov K.M., Pearson D.S., Roels F., Spina C.S., Ebright R., Seligson M., de Soysa Y., Cahan P., Theiβen J., Tu H.C., Han A., Kurek K.C., LaPier G.S., Osborne J.K., Ross S.J., Cesana M., Collins J.J., Berthold F, & Daley G.Q. (2016). Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma. Nature, 535(7611), 246-251.
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5

LIN28B Western Blot Protocol

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Whole-cell lysates for Western blots were extracted with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S). Protein lysates were loaded into Novex 16% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP99165BOX) and separated by gel electrophoresis at 125V for 4 hours. LIN28B and β-actin were detected using an anti-LIN28B antibody (Cell Signaling Technology, #4196) and an anti-ACTB mouse monoclonal antibody (GenScript, A00702), respectively. More details about the antibodies can be found in the Life Sciences Reporting Summary. The Western blot was imaged with Thermo Scientific myECL Imager (Thermo Scientific, 62236).
Jang H.S., Shah N.M., Du A.Y., Dailey Z.Z., Pehrsson E.C., Godoy P.M., Zhang D., Li D., Xing X., Kim S., O’Donnell D., Gordon J.I, & Wang T. (2019). Transposable elements drive widespread expression of oncogenes in human cancers. Nature genetics, 51(4), 611-617.
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