Mouse il 6 elisa kit

The Aβ_1-42 level in brain tissues of mice and the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β were detected by ELISA utilizing mouse Aβ_1-42 ELISA Kit (CUSABIO, Wuhan, China), mouse TNF-α ELISA Kit (Abcam, Cambridge, MA, USA), mouse IL-6 ELISA Kit (Abcam), and mouse IL-1β ELISA Kit (Abcam). For brain tissues, homogenates were centrifuged at 5000 g for 5 min at 4°C, and then supernatant was collected for subsequent assay. For in vitro cells, cell supernatant samples were centrifuged at 500 g for 5 min and the supernatant was collected for subsequent assay. The ELISA was performed in line with the protocols of the manufacturer. The biotinylated antibody (100 μL) was added to each well and incubated at 25°C for 60 min. The horseradish peroxidase (100 μL) was added to each well and incubated away from light at 25°C for 20 min. Termination solution (50 μL) was added to each well, and then the optical density value at 450 nm of each well was detected by a microplate reader (Thermo Fisher Scientific).