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5 protocols using tris hydroxymethyl aminomethane

1

Fabrication of Analytical μPADs

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Whatman filter paper No. 1 was purchased from Whatman International Ltd. (Maidstone, England) and then used for μPADs fabrication. Yellow oily double-sided adhesive tape (brand Youbisheng, width = 40 mm) was purchased from Shengneng Packaging Co., Ltd. (Hangzhou, China). The tape was composed of a double-sided adhesive layer and a protective film. The marker (nib diameter = 0.7 mm), which was used to draw the pattern on the filter paper, was purchased from Shenzhen Shengjie Stationery Wholesale Collection Co., Ltd. Ni(NO3)2,·6H2O, toluene, Cu(NO3)2, Co(NO3)2·6H2O, KNO3, Na2SO4, Ca(NO3)2, Mg(NO3)2, Zn(NO3)2·6H2O, Pb(NO3)2, Fe(NO3)3, CH3COONa, NaF, NaNO2 and MgSO4 of analytical grade were bought from Sinopharm Chemical Reagent Co., Ltd. (China). Mn(NO3)2 was bought from Aladdin Reagent Company (Shanghai, China). Tris(hydroxymethyl)aminomethane was bought from Macklin Biochemical Co., Ltd. (Shanghai, China). The natural mineral water was purchased from Emeishan Yuquan Water Industry Co., Ltd.
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2

Synthesis and Characterization of Dopamine-Functionalized Nanoparticles

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Dopamine hydrochloride (DA), polyvinylpyrrolidone (PVP, Mr = 8000), azobisisobutyronitrile (AIBN), tris(hydroxymethyl)aminomethane (Tris), polyethyleneimine (PEI, Mr = 70 000), N,N′-carbonyldiimidazole (CDI), 1,6-diaminohexane, LTL were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). AgNO3, NaBH4, CPBA, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxy succinimide (NHS), and hydroquinone (HDQ) were obtained from Aladdin Reagent (Shanghai, China). Quercetin (QRT), p-nitrophenol (P-NP) were purchased from Rhawn Chemical Technology (Shanghai, China). Analytical grade sodium phosphate monobasic dihydrate (NaH2PO4), phosphoric acid (H3PO4), styrene (St), methanol (MeOH), ethanol (EtOH), hydrochloric acid (HCl), and sodium hydroxide (NaOH) were purchased from Beijing Chemical Works (Beijing, China). HPLC grade MeOH was purchased from Fisher Scientific (New Jersey, USA).
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3

Synthesis and Characterization of PVDF Membranes

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PVDF membranes with an averaged pore size of 0.22 μm were purchased from Tianjin Jinteng Experimental Equipment Co., Ltd. Dopamine hydrochloride (98%), tris(hydroxymethyl)aminomethane, potassium antimony tartrate (K2Sb2C8H4O12·3H2O, ≥99%) were purchased from Shanghai Macklin Biochemical Co., Ltd. Meanwhile, zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), 2-methylimidazole (Hmim, 98%), potassium dihydrogen phosphate (KH2PO4, ≥99%), sodium carbonate (Na2CO3, ≥98%), sodium sulfate (Na2SO4, ≥99%), sodium chloride (NaCl, ≥99.9%), ammonium tetramolybdate ((NH4)6Mo7O24·4H2O, 99%) were purchased from Shanghai Titan Scientific Co., Ltd. Hydrochloric acid (HCl, 36%), sodium nitrate (NaNO3, ≥99%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Deionized water (DI, R = 18 MΩ cm) was used unless otherwise noted.
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4

Synthesis of Inorganic Compounds

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Tris(hydroxymethyl)aminomethane (C4H11NO3, ≥99.8%), zirconyl chloride octahydrate (ZrOCl2·8H2O, ≥99.9%), zeolite (ZEO, ≥97%) and potassium phosphate monobasic (KH2PO4, ≥99.5%) were purchased from Shanghai Macklin Biochemical Co., Ltd, and hydrochloric acid (HCl 36.0–38.0%), ethyl alcohol (C2H6O, ≥99.5%), sodium hydroxide (NaOH, ≥96%), sodium chloride (NaCl, ≥99.8%), sodium nitrate (NaNO3, ≥99%), sodium sulfate (Na2SO4, ≥99%) and sodium bicarbonate (NaHCO3, ≥99.5%) were obtained from Sinopharm Chemical Reagent Co., Ltd. All reagents were used without any further refinement.
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5

Membrane Interaction of ACE Inhibitory Peptides

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ACE inhibitory tri-peptides ADF, FGR, and MIR were provided by Shanghai Science Peptide Biological Technology Co., Ltd. (Shanghai, China). 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) was obtained from Ponsure Biological (Shanghai, China). 1,6-Diphenyl-1,3,5-hexatriene (DPH), [N,N,N,-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium(p-toluenesulfonate) (TMA-DPH), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (NBD-PE) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Phosphate buffered saline (PBS) (pH 7.4) was obtained from Shanghai Future Industry Co., Ltd. (Shanghai, China). Tris-(hydroxymethyl) aminomethane (≥99%) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). All other chemicals were purchased from commercial sources and of analytical grade.
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