Aniline (C6H7N), poly(2-acrylamido-2-methyl-1-propanesulfonic acid, PAAMPSA, Mw = kD), poly(ethylene glycol), diglycidyl ether (PEDGE, Mw = 500 Da) solution, adenosine 5′-triphosphate disodium salt (ATP) (99%), β-nicotinamide–adenine dinucleotide phosphate (NADP+, >90%), β-nicotinamide-adenine dinucleotide phosphate (reduced form, NADPH, >90%), magnesium chloride hexahydrate (99%), D-gluconic acid sodium salt (99%), sodium phosphate dibasic (Na2HPO4), sodium phosphate monobasic monohydrate (NaH2PO4-H2O) and potassium chloride (KCl) have been purchased from Sigma Aldrich (St. Louis, MO, USA). 6PGDH (EC 1.1.1.44, 150 U/mL) and GK (EC 2.7.1.12, 1500 U/mL) have been purchased from CPC Biotech (CPC Biotech, Napoli, Italy).
β nicotinamide adenine dinucleotide phosphate
Manufactured by Merck Group
Sourced in United States, Germany
β-nicotinamide adenine dinucleotide phosphate (β-NADP) is a coenzyme found in many biological processes. It serves as an electron acceptor and participates in redox reactions, facilitating the transfer of electrons during metabolic pathways.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Glucose 6 phosphate (g6p), by Merck Group (4 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (4 mentions)
Acetonitrile, by Thermo Fisher Scientific (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
Bradford reagent, by Merck Group (3 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (3 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (3 mentions)
Reduced glutathione, by Merck Group (3 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (3 mentions)
Reduced tetra cyclohexyl ammonium salt nadph, by Merck Group (2 mentions)
α naphthyl acetate α na, by Merck Group (2 mentions)
α naphthol, by Merck Group (2 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Glutathione reductase, by Merck Group (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
33 protocols using β nicotinamide adenine dinucleotide phosphate
1
Enzymatic Assay for Gluconate Metabolism
2
Glucose-6-Phosphate Dehydrogenase Assay
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Glucose-6-phosphate (G6P), β-Nicotinamide adenine dinucleotide phosphate (NADP+), β-Nicotinamide adenine dinucleotide phosphate, reduced (NADPH), 6-phosphogluconic acid (6PG), adenosine-5′-triphosphate (ATP), adenosine diphosphate (ADP), β-Nicotinamide adenine dinucleotide (NAD), β-Nicotinamide adenine dinucleotide, reduced (NADH), DL-Dithiothreitol (DTT), nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), blue dextran, bovine serum albumin (BSA), Sephacryl S-300, molecular weight marker kits for gel filtration, diethylaminoethyl-cellulose (DEAE-Cellulose), and chemicals for electrophoresis and isoelectric focusing (IEF) standard markers mixture pI 3.6–9.3 were purchased from Sigma Chemical Co. Molecular weight SDS marker proteins and 2′, 5′ ADP Sepharose 4B were products of Pharmacia Fine Chemicals Co.
3
Cytochrome P450 enzyme inhibition assay
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Sorafenib (purity >98%), β-nicotinamide adenine dinucleotide phosphate (NADP) and Lucifer yellow was purchased from Sigma (St. Louis, MO). Berberine (purity >98%) and triptolide (purity >98%) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Rat liver microsomes were purchased from BD GentestTM (Becton Dickinson, Franklin Lakes, NJ). The Alkaline Phosphatase Assay Kit was provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Formic acid was purchased from Anaqua Chemicals Supply Inc. Limited (Houston, TX). Ultrapure water was prepared with a Milli-Q water purification system (Millipore, Billerica, MA). All other chemicals were of analytical grade or better.
Dulbecco’s modified Eagle’s medium (DMEM) and non-essential amino acid (NEAA) solution were purchased from Thermo Scientific Corp. (Logan, UT). Foetal bovine serum (FBS) was obtained from GIBCO BRL (Grand Island, NY). Penicillin G (10,000 U/mL) and Streptomycin (10 mg/mL) were purchased from Amresco (Solon, OH). Hanks' balanced salt solution (HBSS) was purchased from GIBCO (Grand Island, NY).
Dulbecco’s modified Eagle’s medium (DMEM) and non-essential amino acid (NEAA) solution were purchased from Thermo Scientific Corp. (Logan, UT). Foetal bovine serum (FBS) was obtained from GIBCO BRL (Grand Island, NY). Penicillin G (10,000 U/mL) and Streptomycin (10 mg/mL) were purchased from Amresco (Solon, OH). Hanks' balanced salt solution (HBSS) was purchased from GIBCO (Grand Island, NY).
4
Metabolic Pathways Inhibition Assay
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DSS was purchased from Affymetrix (Cleveland, OH, USA). Nifedipine (NFP, purity≥98%), oxidized NFP (NFPO, purity≥95%), nitrendipine (purity≥95%), diclofenac (DCF) sodium salt, dimethyl sulfoxide (DMSO), Tween 80, β-nicotinamide adenine dinucleotide phosphate, and reduced tetra (cyclohexyl ammonium) salt (NADPH) (purity≥97%) were from Sigma–Aldrich (St. Louis, MO, USA). CsA (purity≥99%), cyclosporine D (purity≥95%), 4′-OH-DCF-13C6, 4′-OH-DCF, 5-OH-DCF, lovastatin hydroxy acid (LVA, purity≥95%), and simvastatin hydroxy acid sodium salt (SVA, purity≥95%) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Lovastatin (LVS, purity≥98%) and pravastatin sodium salt (PVS, purity≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA). The solvents (acetonitrile, methanol and water) for high-performance liquid chromatography (HPLC) were from Thermo Fisher Scientific (Fisher Scientific, Houston, TX, USA).
5
Oxidative Stress Biomarker Quantification
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Reagents were obtained from the following sources: CBD from THC Pharm GmbH (Frankfurt, Germany); 8-iso prostaglandin F2α-d4 (8-isoPGF2α–d4), anandamide-d8 (AEA-d8), 2-arachidonylglycerol-d8 (2-AG-d8), oleoylethanolamide-d4 (OEA-d4), cannabidol-d9 (CBD-d9), leukotriene B4-d4 (LTB4-d4), 5(S)-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic-5,6,8,9,11,12,14,15-d8 acid (15-HETE-d8) and prostaglandin D2-d4 (PGD2-d4) from Cayman Chemical Company (Ann Arbor, MI, USA); α-tocopherol, retinol, L-glutathione reduced (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), catalase (CAT), thioredoxin (Trx), β-nicotinamide adenine dinucleotide phosphate hydrate (NADP), β-nicotinamide adenine dinucleotide phosphate, reduced tetra (cyclohexylammonium) salt (NADPH), hexane, butylated hydroxytoluene (BHT), ethanol, O-(2,3,4,5,6-pentafluoro-benzyl) hydroxylamine hydrochloride (PFBHA-HCl), 4-hydroxynonenal (4-HNE) and benzaldehyde-d6 from Sigma-Aldrich (Saint Louis, MO, USA)
6
Isothiocyanate Compounds and Enzyme Assays
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Allyl isothiocyanate (AITC), 2-phenylethyl isothiocyanate (2PEITC), 3-butenyl isothiocyanate (3BITC) and 3-(methylthio) propyl isothiocyanate (3MPITC) (Figure 2 ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The purities of AITC, 2PEITC,3BITC and 3MPITC were 99.7, 99, 97 and 98%, respectively. The following chemicals were purchased from Sigma-Aldrich as well for enzyme activity assay: Coomassie Brilliant Blue G-250, α-naphthol, β –naphthol, α-naphthyl acetate (α-NA), β-naphthyl acetate (β-NA), β-nicotinamide adenine dinucleotide phosphate (β-NADPH), fast blue B salt, 1-chloro-2,4-dinitrobenzene (CDNB), l -glutathione reduced (GSH), acetylthiocholine iodide (ATC), 5,5’-dithio-bis(2-nitrobenzoic acid) (DTNB).
7
Synthesis and Characterization of ZnO Nanopowder
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Uncoated ZnO nanopowder with an advertised 25-nm particle size was purchased from PlasmaChem GmbH, and ZnCl2 was purchased from Merck Group. Other chemicals used in the present study were of analytical grade unless otherwise stated. Trichloroacetic acid (TCA), Bradford reagent, bovine serum albumin (BSA), glucose standard solution, iodonitrotetrazolium chloride (INT), β-nicotinamide adenine dinucleotide (NADH), β-nicotinamide adenine dinucleotide phosphate (NADPH), polyvinylpyrrolidone (PVP), tris(hydroxymethyl)aminomethane (Tris base), t-octylphenoxypolyethoxyethanol (Triton™ X-100), and glyceryl tripalmitate were purchased from Sigma-Aldrich®. CHCl3 (chloroform), CH3OH (methanol), C6H6O (phenol), NaOH, HCl, H2SO4, MgSO4, and HNO3 (69.9–70.0%; Baker Instra-Analyzed) were purchased from Avantor Performance Materials S.A., and H2O2 (30.0%; Baker Analyzed) was purchased from WITKO®.
8
Bile Acid Profiling by UPLC-QTOFMS
9
Redox Substrate Characterization Protocol
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Electron-acceptor substrates from Sigma Aldrich): coenzyme Q1, coenzyme Q10, DCPIP (2,6-dichloroindophenol sodium salt hydrate), potassium ferricyanide, 1,4-benzoquinone (1,4-BQ), ferrocenium hexafluorophosphate (Fc+), diethyl oxalacetate sodium salt, sodium fumarate dibasic, methyl red sodium (crystalline), 3,3′-methylene-bis(4-hydroxycoumarin). Electron acceptor substrates from Carl Roth (Germany): sodium thiosulphate anhydrous, DL-malic acid. Electron-donor substrates from Sigma Aldrich: β-nicotinamide adenine dinucleotide phosphate and reduced tetra (cyclohexylammonium) salt (NADPH).
10
Cytotoxicity Assay Protocol with Diverse Chemicals
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Chemicals including 1-chloro-2,4-dinitrobenzene (CDNB), 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT), 2,6-dichloroindophenol (2,6-DCIP, sodium salt hydrate), 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), 5-Fluorouracil (5-FU), acetonitrile (can), Bradford Reagent, cumene hydroperoxide, dimethyl sulfoxide (DMSO), flavin adenine dinucleotide disodium (FAD, salt hydrated), glutathione (GSH, reduced form), hydrogen peroxide (H2O2) solution, β-nicotinamide adenine dinucleotide (NAD, reduced disodium salt hydrated), β -nicotinamide adenine dinucleotide phosphate (NADPH), phosphate buffered saline (PBS), sodium chloride, sodium phosphate (NaH2PO4), sulforaphane (SFN), 0.1% aqueous trifluoroacetic acid (TFA) solution, and Trizma® base were purchased from Sigma-Aldrich (Madrid, Spain). Absolute ethanol, di-sodium hydrogen phosphate anhydrous (Na2HPO4), and hydrochloric acid (HCl) were obtained from Panreac AppliChem (Barcelona, Spain). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Munich, Germany).
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