For growth curves, 1 × 104 cells were grown in triplicate in the presence of the indicated treatments. Cells were harvested, stained with trypan blue and live cells were enumerated using a hemocytometer at the indicated timepoints. For studies of cell death, trypan blue exclusion was utilized to quantify cell viability because Q treatment elicits high auto-fluorescence in cells, which precludes the use of fluorescent-based cell death assays. The caspase cleaved cytokeratin 18 (cCK18) M30 fragment was measured using the M30 Cytodeath ELISA (Diapharma P10900), according to the manufacturer’s protocol; this assay quantitatively measures a stable apoptosis-generated neoepitope in CK-18 (Asp396). Levels of reactive oxygen species (ROS) were detected using the ROS-Glo H2O2 luminescence kit (Promega G8820) according to the manufacturer’s protocol.
Ros glo h2o2 luminescence kit
Manufactured by Promega
The ROS-Glo H2O2 luminescence kit is a reagent-based assay designed to detect and quantify hydrogen peroxide (H2O2) levels in various biological samples. The kit utilizes a bioluminescent reaction to generate a luminescent signal proportional to the amount of H2O2 present in the sample.
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Lab products found in correlation
2 protocols using ros glo h2o2 luminescence kit
1
Growth, Viability, and Oxidative Stress Assays
2
Growth, Viability, and Oxidative Stress Assays
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For growth curves, 1 × 104 cells were grown in triplicate in the presence of the indicated treatments. Cells were harvested, stained with trypan blue and live cells were enumerated using a hemocytometer at the indicated timepoints. For studies of cell death, trypan blue exclusion was utilized to quantify cell viability because Q treatment elicits high auto-fluorescence in cells, which precludes the use of fluorescent-based cell death assays. The caspase cleaved cytokeratin 18 (cCK18) M30 fragment was measured using the M30 Cytodeath ELISA (Diapharma P10900), according to the manufacturer’s protocol; this assay quantitatively measures a stable apoptosis-generated neoepitope in CK-18 (Asp396). Levels of reactive oxygen species (ROS) were detected using the ROS-Glo H2O2 luminescence kit (Promega G8820) according to the manufacturer’s protocol.
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