This procedure was reported previously17 (link). Briefly, NPC cells were cultured in 6-well plastic plates at 2 × 105 cells/well and treated with increased doses of β-elemene for 24 h. Afterwards, the cells were harvested, washed with phosphate-buffered saline (PBS), and resuspended in 500 μL of cold PBS and ethanol (1.5 mL) for 2 h at 4 °C. Afterwards, the fixed cells were incubated in 1 mL of 0.1% sodium citrate containing propidium iodide (PI) and RNase for 30 min at room temperature. The cells were washed and subjected to FACS Calibur flow cytometric analysis (FC500, Beckman Coulter, FL, USA), and the proportion of cells within the G0/G1, S, and G2/M phases of the cell cycle were analyzed using the MultiCycle AV DNA Analysis software (Phoenix Flow Systems, Inc. San Diego, CA, USA).
Facs calibur flow
Manufactured by Beckman Coulter
Sourced in United States
The FACS Calibur flow cytometer is a compact and versatile instrument designed for multicolor flow cytometry analysis. It features two lasers, four-color detection, and a high-sensitivity photomultiplier tube (PMT) system. The FACS Calibur is capable of analyzing a wide range of cell types and populations, supporting a variety of applications in life science research and clinical diagnostics.
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Lab products found in correlation
2 protocols using facs calibur flow
1
Cell Cycle Analysis of NPC Cells
2
Shikonin Induces Cell Cycle Alterations
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Hela and c33a cells were cultured in 6-well plates and treated with increased doses of shikonin for 24 h. Cell cycle experiment was carried out using the cell cycle staining kit based on the manufacture’s instruction. In brief, the cells were washed and resuspended in PBS and then incubated with 0.05 mg 0.1% sodium citrate containing propidium iodide and 50 µg RNase for 1 h at room temperature. The cells were washed and subjected to FACSCalibur flow cytometric analysis (FC500, Beckman Coulter, FL, USA). The proportion (percentage) of cells within the G0/G1, S, and G2/M phases of the cell cycle was analyzed using the MultiCycle AV DNA Analysis software (Phoenix Flow Systems, Inc. San Diego, CA, USA).
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