Uv 1601 spectrometer

Manufactured by Shimadzu

Sourced in Japan

The UV-1601 is a compact and versatile UV-Vis spectrometer designed for routine analysis. It features a wavelength range of 190-1100 nm and can perform absorption, transmittance, and absorbance measurements. The UV-1601 employs a double-beam optical system and a deuterium lamp for the UV region and a halogen lamp for the visible region.

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Lab products found in correlation

Labram hr raman spectrometer, by Horiba (1 mentions) Diamond dsc, by PerkinElmer (1 mentions) Vnmrs 500 spectrometer, by Agilent Technologies (1 mentions) Ecosec gpc system, by Tosoh (1 mentions)

Spelling variants of this product

UV-1601 (459 citations) UV-1601 UV-vis spectrometer (7 citations) Spectrometers (2 citations) UV Visible spectrometer 1601 (2 citations) UV-1601 Ultraviolet Spectrometer (2 citations) UV-1601 double-beam spectrometer (2 citations)

5 protocols using uv 1601 spectrometer

SNARF Dye-Protein Interactions at pH

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Different pH buffers were prepared by mixing different amounts of NaOH and KH₂PO₄. The pH level of each sample was confirmed using the micro pH electrode. To study the free SNARF-5F and the SNARF-PAA NP interactions with proteins, HSA (4 mg ml⁻¹) was introduced into different pH buffers containing either free SNARF-5F or SNARF-PAA NPs (2 mg ml⁻¹). Free SNARF-5F dye concentration was equivalent to that loaded in SNARF-PAA NPs. The possible change in the optical absorption property of each sample containing either free SNARF-5F or SNARF-PAA NPs caused by interaction with HSA was measured using the UV-1601 Spectrometer (Shimadzu).

Jo J., Lee C.H., Kopelman R, & Wang X. (2017). In vivo quantitative imaging of tumor pH by nanosonophore assisted multispectral photoacoustic imaging. Nature Communications, 8, 471.

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Characterization of Metal Nanoparticles by Spectroscopy

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IR and Raman samples were prepared by seeding many drops of chloroformic liquors / solutions on a compressed KBr pellet or on a glass slide, respectively; and letting chloroform evaporation to take place at room temperature. Non-volatile compounds contained in the liquors undergo re-precipitation to produce thin layer deposits, which were analyzed by IR and Raman spectroscopy. FTIR spectra were recorded on a Nicolet 5-SXC spectrometer by using a forty scan average and a resolution of 4 cm⁻¹. Raman spectra were recorded on a Horiba Jobin Yvon LabRAM HR Raman spectrometer by using a red laser (632.82 nm) as excitement source. UV-Visible spectra were recorded on a Shimadzu UV-1601 spectrometer by using a 1 cm quartz cell at room temperature to characterize the optical properties of species contained in liquors and the surface plasmon resonance (SPR) of gold (AuNPs) and silver (AgNPs) nanoparticles in chloroform.
Selected experimental samples of AuNPs and AgNPs produced with redox reactions in chloroform were characterized by TEM (JEM-JEOL 1120 microscope). Samples were prepared by using chloroformic solutions containing noble metal NPs without any purification treatment to seed one or two drops of solution onto a carbon-Formvar-covered cooper grid and by allowing for the evaporation of chloroform to occur at room temperature.

Douglas-Gallardo O.A., Gomez C.G., Macchione M.A., Cometto F.P., Coronado E.A., Macagno V.A, & Pérez M.A. (2015). Morphological Evolution of Noble Metal Nanoparticles in Chloroform: Mechanism of Switching on/off by Protic Species. RSC advances, 5(122), 100488-100497.

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Spectrophotometric Determination of Chitosan Degree of Deacetylation

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A Shimadzu UV-1601 spectrometer was used for the ultraviolet-visible spectrophotometry analysis to determine the DD of CHI. First, several solutions of N-acetylglucosamine were dissolved in HCl at various concentrations to obtain a calibration curve. To calculate the DD, the absorbance was measured at 199 nm. The spectra obtained between 200–350 nm were used to confirm the success of conjugating CHI and HA with catechol functional groups. Quantification of the degree of catechol substitution was made via a colorimetric assay and analysis of the absorbance maximum at around 280 nm. HCA and DN solutions were prepared to create a catechol concentration standard curve and quantify the catechol content of CHI–cat and HA–cat, respectively.

Fonseca M.C., Vale A.C., Costa R.R., Reis R.L, & Alves N.M. (2022). Exploiting Polyelectrolyte Complexation for the Development of Adhesive and Bioactive Membranes Envisaging Guided Tissue Regeneration. Journal of Functional Biomaterials, 14(1), 3.

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Characterization of Polymer Materials

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¹ H-NMR spectra were recorded at 500 MHz on an Agilent VNMRS 500 spectrometer at room temperature. Gel permeation chromatography (GPC) measurements were performed on a TOSOH EcoSEC GPC system equipped with an autosampler system, a temperature-controlled pump, a column oven, a refractive index (RI) detector, a purge and degasser unit and TSKgel superhZ2000, 4.6 mm ID x 15 cm x 2 cm column. Tetrahydrofuran was used as an eluent at ﬂow rate of 1.0 mL/min at 40°C. Refractive index detector was calibrated with polystyrene standards having narrow molecular weight distributions. Data were analyzed using Eco-SEC Analysis software. Fourier transform infrared (FTIR) spectra were recorded on Perkin−Elmer FTIR Spectrum One spectrometer with an ATR Accessory and cadmium telluride detector. UV-visible spectra were recorded with a Shimadzu UV-1601 spectrometer. DSC measurements were performed on Perkin-Elmer Diamond DSC with a heating rate of 10°C min⁻¹ under nitrogen flow.

, & Yilmaz G. (2020). In-situ syntheses of graft copolymers by metal-free strategies: combination of photoATRP and ROP. Designed Monomers and Polymers, 23(1), 134-140.

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Determination of Total Polyphenol Content

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Total polyphenol contents of plant extracts were evaluated by the modified Folin-Ciocalteu method [19 (link)]. Na₂CO₃ solution (2 mL, 2%) was added to 0.1 mL extract and mixed for 10 minutes at room temperature. After the vortex, 0.1 mL of 50% Folin-Ciocalteu reagent was added and mixed again for 15 seconds, and then incubated for 30 minutes at 40°C to develop color. The absorbance was measured at 700 nm using UV-1601 spectrometer (Shimadzu, Kyoto, Japan). Plant extracts were evaluated at a final concentration of 1 mg/mL. Total polyphenol content of plant extracts was calculated as quercetin (25 to 500 ppm) using the following equation based on the calibration curve: y = 0.0033x–0.0111, R² = 0.9979, where x was the absorbance and y was the quercetin equivalent (μg/mL) [20 (link)].

Hong H., Lee J.H, & Kim S.K. (2018). Phytochemicals and antioxidant capacity of some tropical edible plants. Asian-Australasian Journal of Animal Sciences, 31(10), 1677-1684.

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