Tyramine hydrochloride

Biogenic amines standards methylamine hydrochloride 99% (MA), ethylamine hydrochloride 98%(EA), dimethylamine hydrochloride 99% (DMA), diethylamine hydrochloride 99% (DEA), propylamine hydrochloride 99% (PA), butylamine 99% (BA), 2-phenylethylamine hydrochloride 98% (PHA), tyramine hydrochloride 98% (TYR), tryptamine hydrochloride 99% (TRP), histamine dihydrochloride 99% (HIS), hexylamine 99%(HEA), isopentylamine 99% (isoPA), putrescine dihydrochloride 98% (PUT), cadaverine dihydrochloride 98% (CAD),spermidine trihydrochloride 99.5%(SPD), spermine tetrahydrochloride 99.5% (SPE), agmatine sulfate 98% (AGM) were obtained from Sigma Aldrich (St. Louis, MO, USA). The internal standard (IS) was 1.7-diamoheptane 98% (DAH) purchased from Sigma Aldrich (St. Louis, MO, USA). Formic acid (FA) was obtained from Merck (Darmstadt, Germany). Boric acid and sodium hydroxide were purchased from POCH (Gliwice, Poland). Acetonitrile (ACN) with LC-MS grade, ammonium formate and tosyl chloride (≥99%) were obtained from Sigma Aldrich (St. Louis, MO, USA). Nylon Captiva Econofilters (25 mm diameter, 0.2 µm pore size) were purchased from Agilent Technologies (Santa Clara, CA, USA). Ultrapure water was produced by the HLP5 system from Hydrolab (Wiślina, Poland).

Sodium alginate (ALG, medium viscosity), tyramine hydrochloride (TYR), N-hydroxysuccinimide (NHS), MES solution, sodium hydroxide (NaOH), HRP, and hydrogen peroxide solution (H₂O₂) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Sodium Chloride (NaCl) was purchased from Daejung (Siheung, Korea). Collagen type I from rat tail (COL) was purchased from Corning Inc. (Corning, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM), penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from Gibco (Waltham, MA, USA).