Saos2

Manufactured by Shanghai Cell Bank

Sourced in China

Saos2 is a human osteosarcoma cell line derived from the primary osteosarcoma of an 11-year-old Caucasian female. It is a widely used model for studying osteosarcoma and bone cell biology.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Saos 2, by Thermo Fisher Scientific (1 mentions) Verapamil, by Selleck Chemicals (1 mentions) Primary antibodies against n cadherin, by Cell Signaling Technology (1 mentions) Nanog, by Proteintech (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) β catenin, by Abcam (1 mentions) Cyclin e, by Proteintech (1 mentions) Hfob1, by Shanghai Cell Bank (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Plko 1 puro vector, by Addgene (1 mentions)

4 protocols using saos2

Comparative Analysis of Osteosarcoma Cell Lines

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MNNG/HOS, U‐2OS, MG‐63, and Saos‐2 were obtained from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). MNNG/HOS and MG‐63 cells were grown in α‐MEM (Hyclone), while Saos‐2 and U‐2OS were cultured in RPMI 1640 medium (Gibco) containing 10% FBS (Gibco) and 1% antibiotics (Penicillin and streptomycin). All the cells were grown at 37°C in a humidified incubator containing 5% CO2.
BD obtained from Shanghai Yuanye Bio‐Technology Co., Ltd (Shanghai, China), was dissolved in DMSO (Sigma‐ Aldrich) and stored at −20°C as single‐use aliquots. BCA protein assay kit was obtained from Beyotime. Matrigel was obtained from BD Bioscience. Verapamil and STAT3 inhibitor Stattic were obtained from Selleck. Primary antibodies against N‐cadherin, GAPDH, β‐actin, and cyclin D1 were purchased from Cell Signaling Technology. Antibodies against MMP‐9, MMP‐2, SHP1, STAT3, phospho‐STAT3 (Y105), JAK2, phospho‐JAK2 (Y1007, Y1008), CD133, Ki‐67, and β‐catenin were purchased from Abcam. Antibodies against Caspase 3, Bcl‐2, CDK2, CDK4, cyclin E, SOX‐2, OCT‐4, and Nanog were purchased from Proteintech.

Wang S., Hu H., Zhong B., Shi D., Qing X., Cheng C., Deng X., Zhang Z, & Shao Z. (2019). Bruceine D inhibits tumor growth and stem cell‐like traits of osteosarcoma through inhibition of STAT3 signaling pathway. Cancer Medicine, 8(17), 7345-7358.

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Osteosarcoma Tissue and Cell Line Analysis

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A total of 18 patients (male, 10; female, 8; age ≤18 years, 13; age >18 years, 5; tumor stage I+II, 15; tumor stage III, 3) diagnosed with OS were recruited from the Second Affiliated Hospital, Chongqing Medical University (Chongqing, China). These patients were divided into two groups (metastasis, vs. no metastasis) according to radiological results. All patients provided written informed consent. The experimental protocols were approved by the Ethics Committee of the Second Affiliated Hospital, Chongqing Medical University. The OS tissues and the corresponding normal tissues (5 cm from the tumor margin) were obtained from resection and then immediately snap frozen in liquid nitrogen for storage at −80°C.
The OS-derived human HOS, Saos2, U2OS and MG-63 cell lines, and the NHOst normal osteoblast cell line were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). The cells were maintained in DMEM with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and incubated in a humidified atmosphere containing 5% CO₂ at 37°C.

Yuan W., Wang D., Liu Y., Tian D., Wang Y., Zhang R., Yin L, & Deng Z. (2017). miR-494 inhibits cell proliferation and metastasis via targeting of CDK6 in osteosarcoma. Molecular Medicine Reports, 16(6), 8627-8634.

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Correlation of circ-0000658 and Osteosarcoma

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Sixty pairs of OS samples were collected from the patients at the China‐Japan Friendship Hospital. All patients provided the written informed consent, and all assay procedures were conducted based on the approval of the Clinical Research Ethics Committees of China‐Japan Friendship Hospital. The correlation between circ‐0000658 expression and the clinical and pathological characteristics of patients is presented in Table 1.
The normal human osteoblast cell line (hFOB1.19) and OS cell lines (HOS, U2OS, SJSA1, Saos2 and MG63) were purchased from Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China). The cells were cultured in DMEM (Dulbecco's modified Eagle's medium; Gibco BRL, Grand Island, NY, USA) containing 10% FBS (Foetal bovine serum; Gibco, Carlsbad, CA, USA) in an incubator (Temp: 37°C; CO₂: 5%).

Jiang X, & Chen D. (2020). Circular RNA hsa_circ_0000658 inhibits osteosarcoma cell proliferation and migration via the miR‐1227/IRF2 axis. Journal of Cellular and Molecular Medicine, 25(1), 510-520.

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Establishment of Osteosarcoma Cell Lines

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Human hFOB1.19 osteoblasts and the osteosarcoma cell lines SAOS2 and MG63 were purchased from Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco's minimal essential medium (DMEM) supplemented with 10% fetal bovine serum (FBS), streptomycin (100 μg/mL), and penicillin (100 U/mL) in a humidified atmosphere with 5% CO₂ at 37°C. The pLKO.1 puro vector (Addgene, Cambridge, MA) with a U6 promoter was used for the construction of recombinant lentiviral plasmid encoding sh‐DRP5. The shRNA sequence was as follows: 5′‐GTGGACGCTTATGAGAAGT‐3′. HEK 293T cells were cotransfected with pLKO.1 puro and packaging vectors. The supernatant was harvested at 48 and 72 h after transfection. MG63 cells were infected with shDRP5 and shCtrl (negative control, NC) in the presence of 5–10 μg/mL polybrene; after 48 h, cells were incubated in medium with 2 μg/mL puromycin for 12 days to generate osteosarcoma‐MG63 shDRP5 stable cell lines.

Wang L., Liu W., Tang H., Xie X., Zou C., Wang Y., Gao Z, & Yin J. (2017). DRP5 is involved in cancer cell growth and predicts poor prognosis in human osteosarcoma. Cancer Medicine, 6(5), 982-993.

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