Advia centaur xp immunoassay system kits

Venous blood samples were obtained by direct venous puncture in the morning after an overnight fast and before any medication was ingested. The serum levels of total T3, total T4, fT4, TSH, anti-TPO, and anti-Tg were measured through the chemiluminescence method using total T3, total T4, fT4, TSH, anti-TPO, and anti-Tg ADVIA Centaur® XP Immunoassay System kits (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA) prior to the Doppler sonography examinations. All the biochemical exams were performed by the laboratory Alta Excelência Diagnóstica-DASA Group, São Paulo, SP, Brazil. The reference values, analytical sensitivities, intra-assay coefficients of variations, and interassay coefficients of variations for the aforementioned assays are as follows, respectively: (A) total T3 = 70–220 ng/mL, 20 ng/dL, 2.22%, and 1.15%; (B) total T4 = 5.1–13.5 μg/dL, 0.3 μg/dL, 2.04%, and 2.98%; (C) fT4 = 0.7–1.8 ng/dL, 0.1 ng/dL, 2.7%, and 2.94%; (D) TSH = 0.4–4.3 μUI/mL (15 to 61 years of age) or 0.4–5.8 μUI/mL (over 61 years of age), 0.008 μUI/mL, 2.02, and 1.55%; (E) anti-TPO < 60 UI/mL, 1.0 UI/mL, 4.1%, and 3.1%; (F) anti-Tg < 60 UI/mL, 1.0 UI/mL, 4.3%, and 3.0%. When serum anti-TPO and anti-Tg levels were below or above the detection limit, the respective minimum and maximum values were adopted as representative for statistical analysis.

Venous blood samples were obtained in the morning, after an overnight fast and before ingesting any medication on the same day that the CDU was performed.
The serum concentrations of total T3, total T4, fT4, TSH, anti-TPO, and anti-Tg were measured through the chemiluminescence method, using total T3, total T4, fT4, TSH, anti-TPO, and anti-Tg ADVIA Centaur® XP Immunoassay System kits (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). All biochemical exams were performed by the High Diagnostic Quality Laboratory (Laboratório Alta Excelência Diagnóstica-DASA Group, São Paulo, SP, Brazil).
The reference values, analytical sensitivities, intra-assay coefficients of variations, and inter-assay coefficients of variations for the aforementioned assays were the following, respectively: (A) total T3 = 70–220 ng/mL, 20 ng/dL, 2.22%, and 1.15%; (B) total T4 = 5.1–13.5 μg/dL, 0.3 μg/dL, 2.04%, and 2.98%; (C) fT4 = 0.7–1.8 ng/dL, 0.1 ng/dL, 2.7%, and 2.94%; (D) TSH = 0.4–4.3 μIU/mL (15–61 years of age) or 0.4–5.8 μIU/mL (over 61 years of age), 0.008 μIU/mL, 2.02%, and 1.55%; (E) anti-TPO < 60 IU/mL, 1.0 IU/mL, 4.1%, and 3.1%; and (F) anti-Tg < 60 IU/mL, 1.0 IU/mL, 4.3%, and 3.0%. When the serum concentrations of anti-TPO and anti-Tg were below or above the detection limit, the corresponding minimum and maximum values were adopted as being representatives for the statistical analysis.