Anti usp49

For western blot assay, cell lysates were added with RIPA buffer with protease and phosphatase inhibitor (Thermo Fisher Scientific, Houston, TX). Following the centrifuging at 12,000 g under 4℃ for 12 min, the supernatants were examined by Western blotting. Antibodies used were as follows: anti‐FABP5 (Cat#:ab37267, abcam, UK), anti‐PDK1 (Cat#:ab52893, abcam, UK), anti‐p‐AKT (T308, Cat#:13038, Cell Signaling Technology), anti‐p‐AKT (Ser473, Cat#:4060S, Cell Signaling Technology), anti‐AKT (Cat#:4691, Cell Signaling Technology), anti‐p‐mTOR (Cat#:ab109268, abcam, UK), anti‐mTOR (Cat#:ab32028, abcam, UK), anti‐ACC1 (Cat#:ab45174), anti‐CD36 (Cat#:ab133625), anti‐VEGFA (Cat#:ab37267, Abcam, UK), anti‐ACOX1 (Cat#:ab184032, Abcam, UK) and anti‐FASN (Cat#:ab128870, Abcam, UK), anti‐USP49 (Proteintech), anti‐FKBP51 (Cat#:ab126715, Abcam, UK) and anti‐GAPDH from (Santa Cruz Biotechnology; Cambridge, MA).

Mouse control IgG (Santa Cruz Biotechnology, sc-2025) and rabbit control IgG (Millipore, 12–370), HRP-conjugated goat-anti mouse or rabbit IgG (Thermo Scientific, PA1-86717 and SA1-9510) (1:3000), HRP-conjugated mouse anti-FLAG (Sigma, A8592)(1:1000), mouse anti-FLAG (Sungene, KM8002)(1:2000), anti-GFP (Sungene, KM8009)(1:2000) anti-β-Actin (KM9001)(1:2000), anti-Tubulin (KM9003), anti-GAPDH(KM9002), anti-HA (COVANCE, MMS-101R)(1:2000), anti-Ubiquitin (sc-8017)(1:500), anti-Ubiquitin K63-specific linkage (Millipore 05–1308)(1:500), rabbit anti-TBK1(Abcam, 96328–11), anti-p-TBK1(Abcam, 109272), anti-IRF3 (sc-9082)(1:1000), anti-p-IRF3 (Cell Singling Technologies, 4947S)(1:1000), anti-IκBα (sc-371)(1:1000), anti-p-IκBα (Cell Singling Technologies, 9246L)(1:1000), anti-USP49 (proteintech,18066-1-AP), anti-mouse MITA and anti-human MITA (Cell Singling Technologies,13647) (proteintech, 19851-1-AP) were purchased from the indicated manufactures. ISD45, DNA90, and HSV120 were previously described [44 (link), 45 (link), 72 (link), 73 (link)]. ISD45: 5’-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3’; DNA90: 5’-TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACATACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA-3’; HSV120: 5’-AGACGGTATATTTTTGCGTTATCACTGTCCCGGATTGGACACGGTCTTGTGGGATAGGCATGCCCAGAAGGCATATTGGGTTAACCCCTTTTTATTTGTGGCGGGTTTTTTGGAGGACTT-3’.