Prism 5 software for windows

Data were expressed as mean ± s.d. and analyzed in the GraphPad Prism 5 Software for Windows (GraphPad Software, San Diego, CA, USA). A statistically significant difference was determined by the Kruskal–Wallis test. Dunn's multiple comparisons test was used if data were not normally distributed. Significant differences were considered when p ≤ 0.05.

Experiments on cell viability assay, western blotting, and real-time quantitative PCR were repeated three times in triplicate measurement. Statistical analyses were performed with one-way ANOVA test followed by a post hoc analysis (Tukey's multiple comparison test) using GraphPad Prism 5 Software for Windows (GraphPad Software, Inc., San Diego, CA, USA). The results were expressed by mean ± standard error of the mean (mean ± SEM) for each group. A P valueless than 0.05 was regarded as statistically significant.

The data were analyzed using the SPSS/PC+ for Windows statistical package (version 23.0; SPSS, Chicago, IL, USA). The Kolmogorov–Smirnov test was used to assess the distribution of variables. Continuous variables were reported as the mean ± SD, while non-continuous variables were reported as the median and 25–75th percentile. The different comparative analyses were performed using a nonparametric Mann–Whitney U test or Kruskal–Wallis test, according to the presence of two or more groups. The strength of the association between the variables was calculated using Pearson’s method (parametric variables) and Spearman’s rho correlation test (nonparametric variables). p < 0.05 was considered statistically significant. GraphPad Prism 5 for Windows software (version 5.03) was used to elaborate the figures, and the statistical analysis of the relative abundance of analyzed genes was carried out the Wilcoxon test.

The data obtained from Experiment 1 were statistically analyzed using GraphPad PRISM 5 for Windows software (GraphPad Software, Inc., La Jolla, CA, USA). Data were analyzed by one-way repeated ANOVA and expressed as mean ± SD for three independent experiments. Statistical significance was determined by Student’s t-test. p-Values < 0.05 were considered statistically significant.

In an attempt to measure the strength of tdTomato-derived fluorescence in the samples (shown in the left panel of Figure 2A) obtained after IPPIGT, the photographic data (corresponding to a 3 mm² portion that had been injected with transposons and contained co-injected TB) were incorporated into a Macintosh computer and processed using a program set with Adobe Photoshop ver. 5 (Adobe System, Inc., Seattle, WA, USA) to quantify the levels of fluorescence. Data were obtained from three injected areas of a pancreas in mice that had been subjected to IPPIGT at different days for each group. The raw data were then normalized to the data obtained from another pancreatic area that had been untransfected. The data were then subjected to statistical analysis using GraphPad PRISM 5 for Windows software (GraphPad software, Inc., La Jolla, CA). Data were analyzed by one-way repeated ANOVA and expressed as mean ± SD. Statistical significance was determined by Student’s t-test. p-Values <0.05 were considered statistically significant.