Quantification of immunofluorescence intensity was achieved with ImageJ (v1.51k). The shape of the cells was outlined and the area, mean gray fluorescence value and integrated density measured. Several background readings were also measured. The “total corrected cellular fluorescence” (=TCCF) was calculated according to following formula: integrated density—(area of selected cell x mean fluorescence of background readings) [63 (link)]. Values of GFP staining were subtracted by values of DAPI staining to obtain cytoplasmic TCCF.
Sc 2012 igg fitc
Sc-2012 IgG-FITC is a fluorescein isothiocyanate (FITC)-conjugated immunoglobulin G (IgG) antibody produced by Santa Cruz Biotechnology. It is designed for use in immunological techniques such as flow cytometry, immunofluorescence, and other applications where a fluorescently labeled IgG is required.
Lab products found in correlation
2 protocols using sc 2012 igg fitc
Immunofluorescence Analysis of GR Expression in MDA-MB-231 Cells
Quantification of immunofluorescence intensity was achieved with ImageJ (v1.51k). The shape of the cells was outlined and the area, mean gray fluorescence value and integrated density measured. Several background readings were also measured. The “total corrected cellular fluorescence” (=TCCF) was calculated according to following formula: integrated density—(area of selected cell x mean fluorescence of background readings) [63 (link)]. Values of GFP staining were subtracted by values of DAPI staining to obtain cytoplasmic TCCF.
Immunofluorescence Microscopy of Glucocorticoid Receptor
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