The determinations of oxidative stress parameters: concentrations of GSH, GSSG, and GSH/GSSG ratio, antioxidant enzyme activities (glutathione peroxidase (GPx) and glutathione reductase (GR)), total antioxidant status (TAS), lipid peroxidation (thiobarbituric acid reactive substances), and the concentrations of cytokines IL-1β, IL6, and TNF-α were measured in the blood of rats by ready-to-use diagnostic kits: GSH/GSSG Ratio Assay Kit (Calbiochem®, a registered trademark of Merck, Darmstadt, Germany), Glutathione Peroxidase Assay Kit and Glutathione Reductase Assay Kit (Cayman Chemical, Ann Arbor, USA), TAS (RANDOX Laboratories Ltd. Crumlin, United Kingdom), TBARS Assay Kit (Cayman Chemical, Ann Arbor, USA), IL-1β (ELISA Kit for Interleukin 1 Beta, Cloud-Clone Corp., USA), IL-6 (Murine IL-6 ELISA Kit Diaclone SAS, France), and TNF-α (Murine TNF-α ELISA kit, Diaclone SAS, France). All diagnostic procedures were performed according to the instructions supplied by the manufacturers of the respective kits.
Murine il 6 elisa kit
Manufactured by Diaclone
Sourced in France, United States
The Murine IL-6 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-6 (IL-6) levels in biological samples. The kit utilizes a specific antibody coated on a microplate to capture IL-6 from the sample, and a second antibody conjugated with a detection enzyme to quantify the bound IL-6. The resulting color change is proportional to the concentration of IL-6 present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Murine tnf α elisa kit, by Diaclone (3 mentions)
Tbars assay kit, by Cayman Chemical (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Pro prep, by iNtRON Biotechnology (1 mentions)
Pge2 high sensitivity elisa kit, by Enzo Life Sciences (1 mentions)
Urinary isoprostane eia kit, by Oxford Biomedical Research (1 mentions)
5 protocols using murine il 6 elisa kit
1
Oxidative Stress and Cytokine Assays in Rats
2
Quantifying IL-6 Levels in Thrombus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the indicated time intervals, thrombus samples were obtained and homogenized with 0.3 ml of PBS (pH 7.2) containing complete Protease Inhibitor Cocktail (Roche Diagnostics). The homogenates were centrifuged at 12,000 g for 15 min. IL-6 levels in the supernatant were measured using a specific ELISA kit (Murine IL-6 ELISA kit, Diaclone, Besancon Cedex, France), according to the manufacturer's instructions. The detection limit was 10 pg/ml. Total protein in the supernatant was measured with a commercial kit (BCA Protein Assay Kit; Pierce) using BSA as a standard. The data were expressed as IL-6 (ng/ml)/total protein (mg/ml) for each sample.
3
Quantifying Proinflammatory Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The circulatory level of proin ammatory cytokines (TNFα, IL-1β, and IL-6) were measured by commercial kits. TNFα level was determined by murine TNFα ELISA kit, IL-1β was determined by IL-1β ELISA kit and IL-6 level was determined by murine IL-6 ELISA kit following the manufacturers protocol (Diaclone SAS, France). For TNFα inter-assay and intra-assay coe cient was 9.4% and 5% respectively and sensitivity was 10.7pg/ml. For IL-1β inter-assay and intra-assay coe cient was > 10% and sensitivity was 4pg/ml. For IL-6 inter-assay and intra-assay coe cient was 7.2% and 7.3% respectively and sensitivity was 10pg/ml.
4
Retinal Cytokine Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh dissected retinas were immediately submerged in Pro-Prep™ (Intron Biotechnology, Seongram, South Korea) after CO2 euthanasia and crushed with a hand shredder. After 2 h, Eppendorf tubes were centrifuged for 15 min at 13,000 r.p.m. Finally, supernatants were collected and stored at − 80 °C until analysed by ELISA.
Each cytokine was measured individually following manufacturer´s instructions using murine TNF-α ELISA kit (Diaclone, Besancon Cedex, France), murine IL-6 ELISA kit (Diaclone), murine TGF-β ELISA kit (Diaclone SAS) and murine PGE2 ELISA kit (R&D Systems, Minneapolis, MN, USA). Absorbances were measured at 450 nm in a spectrophotometer and concentrations calculated from standard curve.
Each cytokine was measured individually following manufacturer´s instructions using murine TNF-α ELISA kit (Diaclone, Besancon Cedex, France), murine IL-6 ELISA kit (Diaclone), murine TGF-β ELISA kit (Diaclone SAS) and murine PGE2 ELISA kit (R&D Systems, Minneapolis, MN, USA). Absorbances were measured at 450 nm in a spectrophotometer and concentrations calculated from standard curve.
5
Quantifying Inflammation Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The urinary F2-isoprostane concentration, the plasma IL-6 concentration and the concentration of PGE2 secreted from intestinal epithelial cells were measured by using commercially available ELISA kits; Urinary Isoprostane EIA kit (Oxford Biomedical Research, Oxford, MI, USA), Murine IL-6 ELISA Kit (Diaclone SAS, Besancon, France) and PGE2 high sensitivity ELISA kit (Enzo Life Sciences, Farmingdale, NY, USA). Each experiment was performed according to the manufacturer’s protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!