Bamh1 xho1

Manufactured by New England Biolabs

BamH1/Xho1 is a restriction enzyme product offered by New England Biolabs. It is a combination of two individual enzymes, BamH1 and Xho1, which recognize and cleave specific DNA sequences. This product can be used for DNA manipulation and analysis applications.

Automatically generated - may contain errors

Lab products found in correlation

Pgex 5x 1 vector, by GE Healthcare (1 mentions) Dh5alpha cells, by Thermo Fisher Scientific (1 mentions) Quikchange primer design, by Agilent Technologies (1 mentions)

2 protocols using bamh1 xho1

Cloning of RBF1 Protein Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols

GST-RBF1-large pocket (LP), GST-RBF1-small pocket (SP), and GST-RBF1-C-terminus (C-term) constructs were generated using the pGEX-5x-1 vector (GE Healthcare Life Sciences) backbone digested with BamH1/Xho1 (NEB). LP, SP, and C-term segments of RBF1 was generated by using cDNA of full length RBF1. BamH1 and Xho1 sites were added to primer ends for successful ligation into vector. Primer sequences used for cloning is the following:
Successfully ligated vectors with appropriate inserts were then transformed into BL21 cells (NEB). Empty pGEX-5x-1 vector was used as a GST-alone control which was transformed into DH5-alpha cells (Thermo Fisher).

Kim M., Tang J.P, & Moon N.S. (2018). An alternatively spliced form affecting the Marked Box domain of Drosophila E2F1 is required for proper cell cycle regulation. PLoS Genetics, 14(2), e1007204.

+ Open protocol

+ Expand

FOXO3 Cysteine Mutation Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

pcDNA3-FLAG, pcDNA3-FLAG-HA, pcDNA3-FLAG-FOXO3, and pcDNA3-FLAG-HA-FOXO3 have previously been described (54 (link)). Site-directed mutagenesis of FOXO3 plasmids was performed using the Stratagene QuikChange II XL kit (Agilent Technologies) following the manufacturer's guidelines. The oligonucleotides used were designed using the online QuikChange Primer design application (Agilent Technologies) and were synthesized from Integrated DNA Technologies. The pcDNA3-FLAG-HA-FOXO3 plasmid was used as a template to generate all five single C-to-S mutants. Each clone obtained was sequenced with four different sequencing primers to span, with overlaps, the entire coding sequence. To generate the double, quadruple, and quintuple C-to-S mutants, newly synthesized single Cys-to-Ser mutants were used as templates for successive mutagenesis rounds with full-length sequencing after each round. To generate the Cys-to-Ser mutants of pcDNA3-FLAG-FOXO3, the inserts of the several C-to-S mutants of pcDNA3-FLAG-HA-FOXO3 were excised with a BamH1-Xho1 (New England Biolabs) restriction enzyme digestion and cloned into the pcDNA3-FLAG-FOXO3 plasmid, also digested with BamH1 and Xho1 to remove the unmutated FOXO3 insert. shRNA for FOXO3 (NM_001455.x-2766s1c1 clone) was purchased from Sigma. shPRDX1 expression constructs were used as previously described (61 (link)).

Hopkins B.L., Nadler M., Skoko J.J., Bertomeu T., Pelosi A., Shafaei P.M., Levine K., Schempf A., Pennarun B., Yang B., Datta D., Bucur O., Ndebele K., Oesterreich S., Yang D., Rizzo M.G., Khosravi-Far R, & Neumann C.A. (2018). A Peroxidase Peroxiredoxin 1-Specific Redox Regulation of the Novel FOXO3 microRNA Target let-7. Antioxidants & Redox Signaling, 28(1), 62-77.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!