P phenylenediamine

Manufactured by Merck Group

Sourced in United States, Canada, Austria, Australia, Germany

P-phenylenediamine is a chemical compound used in various laboratory applications. It is a colorless to brown crystalline solid with a pungent odor. P-phenylenediamine is commonly used as a reagent in analytical chemistry and biochemistry.

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Lab products found in correlation

Hydrochloric acid (hcl), by Merck Group (6 mentions) Methanol, by Merck Group (5 mentions) Acetone, by Merck Group (4 mentions) Ethanol, by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (4 mentions) Provis ax70 microscope, by Olympus (3 mentions) Spurr s resin, by Electron Microscopy Sciences (3 mentions) O dianisidine, by Merck Group (3 mentions) Dimethylformamide, by Merck Group (3 mentions) Aniline, by Merck Group (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Sodium chloride (nacl), by Merck Group (3 mentions) Zen software, by Zeiss (2 mentions) Pyromellitic dianhydride, by Merck Group (2 mentions) Glass coverslip, by Thermo Fisher Scientific (2 mentions) Axioplan 2 microscope, by Zeiss (2 mentions) Plan neo, by Cytiva (2 mentions) Fitc conjugated mouse anti tubulin monoclonal antibody, by Merck Group (2 mentions) Deltavision image restoration microscope, by Cytiva (2 mentions) Hoechst 33342, by Merck Group (2 mentions)

Close spelling variants of this product

TMPD (N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride) N,N-diethyl-p-phenylenediamine N,N,N′,N′-tetramethyl-p-phenylenediamine N′-tetramethyl-p-phenylenediamine N,N-dimethyl-p-phenylenediamine P-Phenylenediamine (p-PDA, , N-dimethyl-p-phenylenediamine P-phenylenediamine (PPD) N,N-Diethyl-p-phenylenediamine sulfate P-phenylenediamine (Pa-1)

70 protocols using p phenylenediamine

Immunofluorescence Staining of Macrophages

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Macrophages were seeded at a density of 10⁵ cells per glass coverslip (12-mm diameter) and fixed for 10 min in 3.7% formaldehyde, washed three times in PBS, and permeabilized for 10 min in PBS containing 0.1% TritonX-100. After three washes with PBS, cells were incubated for 30 min in blocking solution (2% BSA or 2% normal human serum in PBS), washed briefly in PBS, and incubated for 60 min in the primary antibody solution. Cells were washed three times in PBS and then incubated for 30 min in secondary antibody solution. After three washes in PBS, coverslips were mounted on glass slides with Mowiol 4–88 (Roth) containing p-phenylenediamine (Sigma-Aldrich). Images of fixed samples were acquired with a confocal laser-scanning microscope (Leica DMi8 with a TCS SP8 AOBS confocal point scanner) equipped with an oil-immersion 63× HC PL APO Oil CS2 NA 1.40 objective and Leica LAS X SP8 software.

Klose M., Salloum J.E., Gonschior H, & Linder S. (2019). SNX3 drives maturation of Borrelia phagosomes by forming a hub for PI(3)P, Rab5a, and galectin-9. The Journal of Cell Biology, 218(9), 3039-3059.

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Procyanidin B2 Bioassay Protocol

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Procyanidin B2 (≥90% purity) was obtained from Extrasynthese (Genay, France). Glutamate, glycine, PEG-SOD, Hoechst stain, p-phenylenediamine, and monoclonal antibody against β-tubulin were purchased from Sigma-Aldrich (Saint Louis, MO, USA). HA14-1 (2-amino-6-bromo-α-cyano-3-(ethoxycarbonyl)-4H-1-benzopyran-4-acetic acid ethyl ester) and sodium nitroprusside (SNP) were obtained from Calbiochem (San Diego, CA, USA). FITC-conjugated secondary antibody was obtained from Jackson Immunoresearch Laboratories (West Grove, PA, USA). Nitric Oxide Assay kits (EMSNO) were obtained from Thermo Fisher Scientific (Rockford, IL, USA).

Sutcliffe T.C., Winter A.N., Punessen N.C, & Linseman D.A. (2017). Procyanidin B2 Protects Neurons from Oxidative, Nitrosative, and Excitotoxic Stress. Antioxidants, 6(4), 77.

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Immunofluorescent Staining of Tissue Sections

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For immunofluorescent staining, tissue sections were fixed for 8 min in 4% PFA or 2 min in ice-cold acetone (MHCIIA, MHCIIC, Keratin7). Sections were washed in PBS-T containing 0.2% Triton X-100, then incubated in blocking buffer (3% BSA, 5% NGS, 5% NDS in PBS-T) for 15 min. Sections were incubated in primary antibody diluted in blocking buffer for 15 min – 1h at room temperature. After washing in PBS-T, sections were incubated in secondary antibodies for 10 min. Sections were washed, then mounted in 90% glycerol in PBS plus 2.5 mg/ml p-Phenylenediamine (Sigma-Aldrich). The following primary antibodies were used: Rat anti Mouse CD44v6 (Santa Cruz and BioRad), Rabbit anti mouse Pcdh8 (gift from O. Pourquié, Harvard Medical School; (Chal et al., 2017 (link))), rabbit anti MafB (Sigma-Aldrich), rat anti Beta 4 integrin (BD Biosciences), mouse anti keratin 7 (Abcam), rabbit anti MHCIIC (Biolegend), rabbit anti MHCIIA (Biolegend), rabbit anti phospho-MLC (18/19) (Cell Signaling Technology), rat anti alpha 6 integrin (BD Biosciences), rabbit anti alpha-catenin (Sigma-Aldrich), rabbit anti Ki67 (Abcam), mouse anti NPC1L1 (Santa Cruz), and goat anti CD36 (R&D Systems). Tissue sections were imaged on a Zeiss AxioImager Z1 microscope with Apotome.2 attachment, Plan-APOCHROMAT 20×/0.8 objective or Plan-NEOFLUAR 40×/1.3 oil objective, Axiocam 506 mono camera, and Zen software (Zeiss).

Sumigray K.D., Terwilliger M, & Lechler T. (2018). Morphogenesis and Compartmentalization of the Intestinal Crypt. Developmental cell, 45(2), 183-197.e5.

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Chromosome Spreads and FISH Mapping

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Chromosome spreads were performed as described previously (Fung et al., 2004 (link)). Fluorescent in situ hybridization (FISH) was carried out using two adjacent interval-specific DNA probes. For the LEU2-MAT interval, the plasmid 12B (Newlon et al., 1991 (link)) containing a 20-kb region of chromosome III extending ∼5 kb centromere-distal and ∼15 kb centromere-proximal of the RPS14A gene was used to make probe. For the HIS4-LEU2 interval, a 15-kb region starting at HIS4 and ending in the middle of KCC4 was PCR-amplified from genomic DNA in 2-kb segments. Probes were labeled with biotin-14-dATP (Invitrogen # 19524016, Waltham, MA) or digoxygenin-11-dUTP (Roche #11093088910, Basel, CH) and hybridization was performed as described in Dernburg and Sedat (Dernburg and Sedat, 1998 (link)). Slides were stained with anti-rabbit Zip1 antibody and then with secondary antibodies: rhodamine anti-DIG and FITC-streptavidin and Cy5 anti-rabbit antibody. To stain DNA, 1 μg/mL DAPI was added to the mount made from 0.1% p-phenylenediamine (Sigma Aldrich #P6001, Burlington, MA) in glycerol. Zip1 polyclonal antibodies were generously provided by G.S. Roeder (Yale University).

Pollard M.G., Rockmill B., Oke A., Anderson C.M, & Fung J.C. (2023). Kinetic analysis of synaptonemal complex dynamics during meiosis of yeast Saccharomyces cerevisiae reveals biphasic growth and abortive disassembly. Frontiers in Cell and Developmental Biology, 11, 1098468.

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Synthesis of Fluorescent Probe for Macrophages

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p-Phenylenediamine, 4-formylbenzeneboronic acid, and H₂O₂ were purchased from Sigma-Aldrich. The RAW 264.7 macrophage cell line was purchased from NCCS Pune. Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich. ProLong Gold Antifade Mountant with DAPI was obtained from Thermo Fisher Scientific, and Fluoromount aqueous mounting medium and paraformaldehyde were from Merck. The other reagents for the experiments were of analytical grade and utilized as received. Ultrapure water was produced from a Millipore-Q water purification system.

Phukan K., Sarma R.R., Dash S., Devi R, & Chowdhury D. (2021). Carbon dot based nucleus targeted fluorescence imaging and detection of nuclear hydrogen peroxide in living cells. Nanoscale Advances, 4(1), 138-149.

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Synthesis of Polyimide Precursors

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Pyromellitic dianhydride, 4,4′-diaminodiphenyl ether, m-phenylenediamine, 3,4′-diaminodiphenyl ether, p-phenylenediamine, 5,5′-((propane-2,2-diylbis(4,1-phenylene))bis(oxy))bis(isobenzofuran-1,3-dione), and 4,4′-diaminodiphenylmethane were purchased from Sigma-Aldrich. α,α′-Bis(4-aminophenyl)−1,4-diisopropylbenzene, 4-bis(4-aminophenoxy)-benzene, 4,4′-(hexafluoroisopropylidene)diphthalic anhydride and 4,4′-oxydiphthalic anhydride were purchased from TCI. 2-Methyl-m-phenylenediamine and 1,2,4,5-cyclohexanetetracarboxylic dianhydride were provided by Anhui Zesheng Technology Co., Ltd.

Wang R., Zhu Y., Fu J., Yang M., Ran Z., Li J., Li M., Hu J., He J, & Li Q. (2023). Designing tailored combinations of structural units in polymer dielectrics for high-temperature capacitive energy storage. Nature Communications, 14, 2406.

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Microscopic Analysis of Microtubule Dynamics

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All experiments were performed on a Zeiss Axioplan2 microscope (Carl Zeiss MicroImaging), with a 40x objective (Plan Neo, NA 0.75) and DeltaVision Image Restoration Microscope (Applied Precision). HCT116 cells were plated on glass coverslips (Fisher Scientific) in 6-well dishes 24 hours before fixation. Cells were exposed to DMSO only (vehicle control), ispinesib (50 and 100 nM) for 4 hours, or 50 ng/mL (166 nM) nocodazole for 14 hours at 37 °C in complete media. Cells were fixed for 10 min at 37°C in fix solution (4% formaldehyde, 0.2% Triton X-100, 10 mM EGTA, 1 mM MgCl2, 100 mM PIPES pH 6.8). Coverslips were washed 3 times with TBS-tx (TBS + 0.1% Triton X-100), blocked with AbDil (2% BSA in TBS-tx buffer) and incubated for 1 hr at room temperature with FITC-conjugated mouse anti-tubulin monoclonal antibody (Sigma # F2168; 1:2000 dilution in AbDil). Coverslips were washed three times in TBS-tx, and DNA was stained with Hoechst 33342 (Sigma; 1:10,000). Coverslips were mounted in 0.5% p-phenylenediamine (Sigma) in 20 mM Tris, at pH 8.8, with 90% glycerol and sealed with nail polish.

Kasap C., Elemento O, & Kapoor T.M. (2014). DrugTargetSeqR: a genomics- and CRISPR/Cas9-based method to analyze drug targets. Nature chemical biology, 10(8), 626-628.

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Immunofluorescence Staining of Transfected U2OS Cells

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Transfected U2OS cells were fixed with 4% formaldehyde in 6 well μ-slides (Ibidi) 1× PBS for 30 minutes at 25°C and subsequently washed with ~200 μl PBS three times. Cells were then permeabilized with 0.2% Triton X-100 in PBS for 10 minutes and washed with ~200 μl PBS three times. Cells were blocked with 1% BSA in PBS for 10 minutes, washed with PBS three times, and then incubated with primary antibody for 1 h at 25°C. Cells were washed with PBS three times, and then stained with 300nM DAPI for 10 minutes. The cells were again washed with PBS three times and incubated with FITC-conjugated goat anti-mouse IgG for 30 minutes. Cells were washed with 1× PBS three times and visualized in 0.5 % pphenylenediamine (Sigma) in 20 mM Tris, pH 8.8 with 90 % glycerol. Cells were quantified using ImageJ and color was applied using Adobe Photoshop CS3. PHH3 staining was done with an anti Ser-10 histone H3 antibody (sc-8656-R, Santa Cruz Biotechnology). Validation for sc-8656-R is available from Santa Cruz Biotechnology.

Campbell Z.T., Valley C.T, & Wickens M. (2014). A protein-RNA specificity code enables targeted activation of an endogenous human transcript. Nature structural & molecular biology, 21(8), 732-738.

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Synthesis of Polyimide Precursors

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3,3′,4,4′-Biphenyltetracarboxylic dianhydride (BPDA, ≥99.7%) and 4, 4′-oxydianiline (ODA, ≥99.6%) were supplied by Changzhou Sunlight Pharmaceutical Co., Ltd., (Changzhou, China). p-Phenylenediamine (PDA) was purchased from Sigma-Aldrich (Shanghai, China). N,N-Dimethylformamide (DMF) with analytical grade was provided by Shanghai Ling Feng Chemicals Co., Ltd., (Shanghai, China) and stored over 4 Å activated molecular sieves for at least a week prior to use.

Li J., Song G., Yu J., Wang Y., Zhu J, & Hu Z. (2017). Preparation of Solution Blown Polyamic Acid Nanofibers and Their Imidization into Polyimide Nanofiber Mats. Nanomaterials, 7(11), 395.

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Immunofluorescence Staining of Cultured Cells

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Cells were seeded on poly-L-lysine coated coverslips (BD biosciences) 24–48 h prior to the experiment. Coverslips were washed in PBS and fixed in 1 mL of 3% paraformaldehyde for 15 min at room temperature followed by a PBS wash then permeabilized with 1 mL of 0.5% Triton-X-100 solution. For FK2 staining, cells were pre-extracted with 0.5% Triton-X 100 solution for 10 s prior to fixation. Samples were then washed again in 1× PBS and incubated with primary antibodies (1:500 in 3% BSA) for 1 h, followed by a PBS wash. Coverslips were then incubated with secondary antibodies (1:500 in 3% BSA) and DAPI (200 µg/mL) for 30 min at room temperature protected from light. Samples were then mounted onto glass slides with an anti-fade solution (0.02% p-phenylenediamine [Sigma, P6001] in 90% glycerol in PBS). Samples were visualized and captured using a Ti-2 inverted C2 + confocal microscope.

Kelliher J.L., West K.L., Gong Q, & Leung J.W. (2020). Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination. Nature Communications, 11, 2462.

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