Cryo-EM grids were pre-screened in either a JEOL 1230 and FEI Talos Artica microscope equipped with a TemCam-F416 (TVIPS, Gauting, Germany) or Falcon III (FEI) camera, respectively. High-resolution data were collected on a Titan Krios electron microscope operated at 300 kV, and images were acquired using a Gatan K2 Summit direct electron detector, operated in electron counting mode. For the apo sample, EPU (FEI) was used to record 3161 micrographs at a nominal defocus range of −1.2 to −2.7 μm, with a magnification of 1.07 Å/px at the specimen level, and a total dose of 61 e-/A2 accumulated over 10 seconds and fractionated across 40 frames. For the DnaBC-ssDNA complex, 1858 micrographs were collected at a nominal defocus range of −1.2 to −2.7 μm, with a magnification of 1.047 Å/px at the specimen level, and a total dose of 58 e-/A2 accumulated over 12 seconds and fractionated across 40 frames.
Talos artica
Manufactured by TVIPS
Sourced in Germany
The Talos Artica is a transmission electron microscope (TEM) designed for cryogenic imaging applications. It features a stable and reliable cryo-stage capable of maintaining sample temperatures at or below -170°C. The Talos Artica is optimized for high-resolution imaging of sensitive biological samples in their native hydrated state.
Automatically generated - may contain errors
2 protocols using talos artica
1
Cryo-EM Structure Determination Workflow
2
Cryo-EM Structural Analysis of Vip3Aa
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Cryo-EM grids were pre-screened in a JEOL 1230 and in a FEI Talos Artica microscopes equipped with a TemCam-F416 (TVIPS, Gauting, Germany) and a Falcon III (FEI) camera, respectively. High-resolution data were collected on a Titan Krios electron microscope operated at 300 kV, and images were acquired using a Gatan K2 Summit direct electron detector, operated in electron counting mode. For undigested Vip3Aa, EPU (Thermo) was used to record 3511 micrographs at a defocus range of −1.0 to −3.0 μm, with a magnification of 1.048 Å px−1 at the specimen level, and a total dose of 56.6 e- A−2 accumulated over 12 s and fractionated across 40 frames. For the digested Vip3Aa, 3090 micrographs were collected at a defocus range of −1.0 to −2.9 μm, with a magnification of 1.055 Å px−1 at the specimen level, and a total dose of 60 e- A−2 accumulated over 11 s and fractionated across 44 frames.
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