Anti suz12 d39f6

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-Suz12 D39F6 is a rabbit monoclonal antibody that recognizes the Suz12 protein. Suz12 is a core component of the Polycomb Repressive Complex 2 (PRC2), which is involved in the epigenetic regulation of gene expression.

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Lab products found in correlation

Anti ezh2 ac22, by Cell Signaling Technology (1 mentions) Anti sox2 af2018, by R&D Systems (1 mentions) Anti flag, by Merck Group (1 mentions) Ab9049, by Abcam (1 mentions) Anti h3k27me3, by Cell Signaling Technology (1 mentions) Sc 32921, by Santa Cruz Biotechnology (1 mentions) Anti h2ak119ub1, by Cell Signaling Technology (1 mentions) Anti jarid2, by Cell Signaling Technology (1 mentions) Anti aebp2, by Cell Signaling Technology (1 mentions) Ab201510, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Bca protein assay kit, by Biosharp (1 mentions) Active rap1 detection kit, by Cell Signaling Technology (1 mentions) Anti p erk sc 7383, by Santa Cruz Biotechnology (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)

Spelling variants of this product

SUZ12 (51 citations) Anti-SUZ12 (22 citations) SUZ12 (D39F6) (3 citations)

4 protocols using anti suz12 d39f6

Comprehensive Antibody Inventory for Molecular Biology

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The antibodies used in this work are purchased by Santa Cruz Biotechnology (anti-MYC sc-764 for immunoprecipitation assay, ChIP experiments and immunoblotting; anti-N-MYC sc-56729; anti-MAX sc-197 and sc-765; anti-E-Cadherin sc-7870; anti-Oct3/4 sc-5279; anti-GST sc-138; anti-His sc-8036; anti-Tle3 sc-9124; anti-Tle4 sc-365406; anti-Tcf-3 sc-8635; anti-ERα sc-544), by Millipore (anti-histone H3 06-755; anti-trimethyl histone H3 Lys4 07-473; anti-trimethyl histone H3 Lys27 07-449; anti-Eed 05-1320; anti-Ezh2 07-689), by Cell Signalling (anti-Suz12 D39F6; anti-Ezh2 AC22; Phospho-GSK-3β (Ser9) 9323; anti-GSK-3β 9315; anti Lrp6 3395; anti-Ph-Lrp6 2568; anti-Phospho-Akt (Ser473) 4060; anti-Phospho-Akt (Thr308) 2965; anti-Akt 2920; anti-Erk1/2 9102; anti-Ph-Erk1/2 4370; anti-Active β-catenin, 8814; anti-Axin1 2087; anti-Tcf1 2203) by Abcam (anti-Myc ab11917 for MycER immunoblotting; anti-Suz12 ab12073; anti-Aebp2 ab101655; anti-Utf1 ab24273) and by Bethyl laboratories (anti-Menin A300-105A; anti Ash2l A300-112A; anti-Rbbp5 A300-109A; anti-Nanog A300-397A), by BD Trasduction Laborotories (anti-β-catenin 610153); from Zymed anti-Lamin B1 33-2000; from R&D (anti-Sox2 AF2018; anti-Klf4 3158) and anti-Ring1B was provided by Dr Haruhiko Koseki (RIKEN Yokohama Institute, Japan).

Fagnocchi L., Cherubini A., Hatsuda H., Fasciani A., Mazzoleni S., Poli V., Berno V., Rossi R.L., Reinbold R., Endele M., Schroeder T., Rocchigiani M., Szkarłat Ż., Oliviero S., Dalton S, & Zippo A. (2016). A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nature Communications, 7, 11903.

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Comprehensive Epigenetic Regulation Analysis

Cited 1 time

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Western blot and ChIP analyses were performed with: anti-BAP1 (D7W70; Cell Signaling), anti-Suz12 (D39F6; Cell Signaling Technology), anti-FLAG (F3165; Sigma-Aldrich), anti-H3K27me3 (9733; Cell Signaling Technology), anti-H2AK119ub1 (8240; Cell Signaling Technology), anti-H3 (ab1791; Abcam), anti-H2A (07-146; Sigma-Aldrich), anti-RING1B (homemade (Chiacchiera et al., 2016 (link))), anti-PCGF1, anti-PCGF2, and anti-PCGF6 (Pasini’s lab, homemade (Scelfo et al., 2019 (link))), anti-JARID2 (13594, Cell Signaling Technology), anti-AEBP2 (14129; Cell signaling Technology), anti-HA (C29F4, Cell Signaling Technology), anti-ASXL2 (A302-037A, Bethyl), anti-ASXL1 (D1B6V, Cell Signaling Technology) anti-KDM1B/LSD2 (E1R60, Cell Signaling Technology), anti-OGT (SC-32921, Santa-Cruz), anti-HCFC1 (A301-400A, Bethyl), anti-FOXK2 (A301-730A, Bethyl), anti-MBD6 (SAB1305225-40TST, Sigma-Aldrich), anti-H3K27ac (AB4729, Abcam) anti-PCGF3/5 (AB201510, Abcam), anti-UTX (Helin lab, homemade (Agger et al., 2007 (link))), anti-MTF2 (Proteintech 16208-1-AP), anti-CBX7 (abcam, ab21873), anti-RYBP/DEDAF (Sigma-Aldrich, PRS2227), anti-H3K36me2 (abcam, ab9049), anti-RING1B-pS168 (abcam, ab234421).

Conway E., Rossi F., Fernandez-Perez D., Ponzo E., Ferrari K.J., Zanotti M., Manganaro D., Rodighiero S., Tamburri S, & Pasini D. (2021). BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation. Molecular Cell, 81(17), 3526-3541.e8.

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Protein Expression and Activation Analysis

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Total cell lysates were prepared using RIPA lysis buffer supplemented with PMSF (Solarbio, China). The protein concentration was determined using a BCA protein assay kit (Biosharp, China). Proteins were resolved by SDS-PAGE and electrotransferred to PVDF membranes (Millipore, USA). Anti-ERK2 (sc-1647), anti-adenylate cyclase 1 (sc-365350), and anti-p-ERK (sc-7383) antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, USA). Anti-SUZ12 (D39F6) antibodies were purchased from Cell Signaling Technology (CST, USA). The Rap1 activation assay was performed with an Active Rap1 Detection Kit (Cell Signaling Technology, USA) according to the instructions.

Li W., Hu C., Zhang X., Wang B., Li Z., Ling M., Sun S., Guo C., Li D, & Liu S. (2021). SUZ12 Loss Amplifies the Ras/ERK Pathway by Activating Adenylate Cyclase 1 in NF1-Associated Neurofibromas. Frontiers in Oncology, 11, 738300.

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Immunophenotyping and Western Blot Analysis

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Anti-CD4 PE (RM4-5), anti-CD8a PECy7 (53-6.7), anti-TCRβ FITC (H57-597), anti-CD3 APC (17A2) and the respective isotype controls were purchased from Biolegend (Ozyme, Saint-Quentin-en-Yvelines, France). Anti-GAPDH (G9545) and anti-EZH2 (07-689) used for Western blot, and anti-EED (AA19), anti-H3K27me3 (07-449), anti-H3K4me2 (07-030) and anti-H3K9ac (07-352) antibodies were purchased from Merck Millipore (St.-Quentin-en-Yvelines, France). Anti-Suz12 (D39F6) and anti-H3K9-panmethyl (4069) antibodies were purchased from Cell Signaling (Danvers, MA, USA). The anti-γH2A.X (39118) and the anti-H3 C-terminal (39164) antibodies were obtained from Active Motif (La Hulpe, Belgium). Anti-PARP1 antibody (GTX100573) came from Gentex (Zeeland, MI, USA). The EZH2 inhibitor GSK126 was purchased from Interchim (Montluçon, France) (Table S1).
The SCIET27 cell line was kindly provided by I. Screpanti (Laboratory of Molecular Pathology, Sapienza University of Rome, Rome, Italy) and I. Aifantis (New York University, School of Medicine, New York, NY, USA) (Table S1).

Calcagno G., Ouzren N., Kaminski S., Ghislin S, & Frippiat J.P. (2022). Chronic Hypergravity Induces a Modification of Histone H3 Lysine 27 Trimethylation at TCRβ Locus in Murine Thymocytes. International Journal of Molecular Sciences, 23(13), 7133.

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