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Stainless steel grid shock floor

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The stainless steel grid shock floor is a laboratory equipment designed to provide a controlled electrical stimulus to small animals in a research setting. It consists of a stainless steel grid that can be connected to an electrical source, allowing for the delivery of a mild shock. The core function of this product is to facilitate the study of animal behavior and physiological responses in a controlled environment.

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Freezeframe, by Harvard Apparatus (3 mentions) Freezeview, by Harvard Apparatus (3 mentions)

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5 protocols using stainless steel grid shock floor

1

Inescapable Stress Induces Behavioral Changes

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Rats were placed in a 30.5 × 25.4 × 30.5 cm3 square conditioning chamber with metal walls and a stainless-steel grid shock floor (Coulbourn Instruments, Whitehall, PA, USA). Rats randomly assigned to the stress group received a two-day inescapable shock treatment, where each day they received 60 × 15 s 0.8 mA foot shocks with an inter-trial interval (ITI) of 30 s with a 25% deviation (+/− 7.5 s). Control rats were handled daily but not exposed to conditioning chambers. Electrophysiological experiments and PPI assays were conducted 24 h following inescapable stress. MK-801 induced locomotor response assays were conducted 48 h following inescapable stress.
Elam H.B., Perez S.M., Donegan J.J, & Lodge D.J. (2021). Orexin receptor antagonists reverse aberrant dopamine neuron activity and related behaviors in a rodent model of stress-induced psychosis. Translational Psychiatry, 11, 114.
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2

Fear Conditioning in Rats: Tone Pre-exposure

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Female MAM- and saline-treated rats were placed into a 30.5 × 25.4 × 30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments, Whitehall, PA, USA). Rats were randomly assigned into two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 second tone over 16 trials with a pseudo-random ITI averaging 2 minutes. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which a 20 second tone co-terminated with a mild (0.8 mA, 0.5 sec) foot shock. The tone-shock pairing was presented 4 times with a pseudo-random ITI averaging 2 minutes. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 second tone over 4 trials. Behavior was video recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 second.
Perez S.M., Donegan J.J, & Lodge D.J. (2019). Effect of estrous cycle on schizophrenia-like behaviors in MAM exposed rats. Behavioural brain research, 362, 258-265.
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3

Latent Inhibition Test for Schizophrenia

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The latent inhibition test was used to model a positive symptom of schizophrenia. Briefly, rats were placed into a 30.5×25.4×30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments) and randomly assigned to two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 sec tone over 16 trials with a pseudorandom intertrial interval averaging 2 min. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which the 20 sec tone co-terminated with a mild (0.8 mA, 0.5 sec) footshock. The tone-shock pairing was presented 4 times with a pseudorandom intertrial interval averaging 2 min. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 sec tone over 4 trials. Behavior was video-recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 sec. The percentage of latent inhibition was determined using the following formula: % LI = 100 − NoTone/Tone x 100.
Donegan J.J., Tyson J.A., Branch S.Y., Beckstead M.J., Anderson S.A, & Lodge D.J. (2016). Stem cell derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Molecular psychiatry, 22(10), 1492-1501.
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4

Latent Inhibition Test for Schizophrenia

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The latent inhibition test was used to model a positive symptom of schizophrenia. Briefly, rats were placed into a 30.5×25.4×30.5 cm3 square conditioning chamber with metal walls and a stainless steel grid shock floor (Coulbourn Instruments) and randomly assigned to two groups: tone pre-exposed and non-exposed. Pre-exposed rats were presented with a 20 sec tone over 16 trials with a pseudorandom intertrial interval averaging 2 min. Immediately following tone exposure, rats underwent an established fear conditioning procedure in which the 20 sec tone co-terminated with a mild (0.8 mA, 0.5 sec) footshock. The tone-shock pairing was presented 4 times with a pseudorandom intertrial interval averaging 2 min. Twenty-four hours after conditioning, rats were returned to the conditioning chamber and re-exposed to the 20 sec tone over 4 trials. Behavior was video-recorded at each stage and freezing behavior was analyzed off-line using FreezeFrame and FreezeView software (Coulbourn Instruments). Freezing was defined as behavior below a motion index threshold of 10 lasting at least 1 sec. The percentage of latent inhibition was determined using the following formula: % LI = 100 − NoTone/Tone x 100.
Donegan J.J., Tyson J.A., Branch S.Y., Beckstead M.J., Anderson S.A, & Lodge D.J. (2016). Stem cell derived interneuron transplants as a treatment for schizophrenia: preclinical validation in a rodent model. Molecular psychiatry, 22(10), 1492-1501.
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5

Inescapable Foot Shock Stress in Rats

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Adult rats were randomly assigned to control (no shock) or shock groups who received 2 consecutive days of inescapable foot shock stress. The 2-day IS paradigm consists of rats placed in a 30.5 × 25.4 × 30.5 cm3 square conditioning chamber with a stainless-steel grid shock floor (Coulbourn Instruments, Whitehall, PA, USA). One session of IS consisted of 60 × 15-second, 0.8-mA foot shocks with an average inter-trial interval of 30 seconds with a 25% deviation (±7.5 seconds) and lasts approximately 40 minutes. Control rats were handled daily but not exposed to conditioning chambers. Rats were further assigned to specific treatment groups (n = 5–6 electrophysiology, n = 8–12 PPI). Electrophysiology and behavioral experiments were conducted 24–48 hours after the last day of IS.
McCoy A.M., Prevot T.D., Mian M.Y., Cook J.M., Frazer A., Sibille E.L., Carreno F.R, & Lodge D.J. (2022). Positive Allosteric Modulation of α5-GABAA Receptors Reverses Stress-Induced Alterations in Dopamine System Function and Prepulse Inhibition of Startle. International Journal of Neuropsychopharmacology, 25(8), 688-698.
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