Hs746t

Five gastric cancer cell lines (SNU5, SNU620, SNU638, Hs746T, and MKN45) were obtained from the Korean Cell Line Bank. Normal immortalized cell lines (HFE145) were obtained from Dr. Ashktorab (Howard University, Washington, DC, USA). Two non-small cell lung cancer (NSCLC) cell lines, EBC-1 and H1993, were purchased from the Japanese Research Resources Bank and the American Type Culture Collection, respectively. All cell lines were authenticated by short tandem repeat analyses in Korea Genome Information Institute and were checked for mycoplasma using the e-Myco VALID Mycoplasma PCR Detection Kit (#25245; iNtRon Biotechnology, Inc., Seongnam-si, Korea).

All cell lines used in this paper, except Hs746T, were purchased from Korean Cell Line Bank (KCLB, Korea). Hs746T cell line was purchased from ATCC. These are all gastric adenocarcinoma cells. SNU-5, SNU-620, SNU-638, MKN-45, and Hs746T cell lines show high expression of c-Met, whereas others show low level of c-Met. These cell lines were maintained in RPMI 1640 medium supplemented with 10 % FBS (HyClone, US) using a humidified incubator with 5 % CO2 at 37 °C.

Human gastric carcinoma cell lines (NCI-N87, YCC-19, YCC-38, KATOIII, Hs746T, and MKN74), normal gastric epithelial cell line (HFE145), and normal primary human umbilical vein endothelial cell line (HUVEC) were used in this study. NCI-N87 and HUVEC cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, United States). KATOIII, Hs746T, and MKN74 cell lines were purchased from Korean Cell Line Bank (KCLB; Seoul, Korea). YCC-19 and YCC-38 cell lines were established and provided by Sun Young Rha (Yensei University, Seoul, Korea) (Kim et al., 2018 (link)). HFE145 cell line was provided by Hassan Ashktorab (Howard University, MD, United States) (Marlink et al., 2003 (link)). All cell lines were cultured with RPMI 1640 Medium (Hyclone, Logan, UT, United States) supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT, United States), 100 units/ml penicillin, and 100 μg/ml streptomycin (Hyclone, Logan, UT, United States). HUVECs were maintained in VascuLife EnGS (containing endothelial cell growth supplement; Lifeline Cell Technology, Frederick, MD, United States). All cultured cells were incubated at 37°C in a cell culture incubator with 5% CO₂.