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Annexin 5 fitc pi assay kit

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Annexin V FITC/PI assay kit is a laboratory reagent used to detect and quantify apoptosis in cell samples. It contains Annexin V labeled with the fluorescent dye FITC and the nuclear stain propidium iodide (PI). The kit enables the identification of early and late stage apoptotic cells through flow cytometry or fluorescence microscopy.

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2 protocols using annexin 5 fitc pi assay kit

1

Reagents and Kits for Cell Experiments

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Opti-MEM medium and Lipofectamine 2000 (Lipo) were obtained from the Gibco-Invitrogen Corporation (USA). Trypsin was obtained from the HyClone Corporation (USA). The β-actin mAb was purchased from the Shanghai Kangcheng Biotechnology Corporation (Shanghai, China). The SnoN mAb was purchased from the Abcam Corporation (UK). The cDNA synthesis kit and TRIzol kit were purchased from Bao Biotechnology Corporation (Dalian, China). The RNA PCR kit was obtained from TaKaRa Corporation (Japan). The Western Blotting Detection system was obtained from Thermo Corporation (USA). The cell lysate and bicinchoninic acid (BCA) protein assay kit was purchased from Shanghai Biyuntian Biotechnology Corporation (Shanghai, China). The Cell Counting Kit-8 was obtained from Dojindo Chemical Corporation (CCK-8, Japan). The Annexin V FITC/PI assay kit was purchased from Invitrogen Corporation (USA).
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2

Evaluating Apoptosis and Oxidative Stress

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ISO was obtained from Sigma Biotechnology (Sigma, USA). Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay kit was purchased from Beyotime Biotechnology (Shang Hai, China). AnnexinV-FITC/PI assay kit was obtained from Invitrogen (Eugene, USA). Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), malondialdehyde (MDA) assay kit were purchased from Naning Jiancheng Institue of Biological Engineering (Nanjing, China). All antibodies were acquired from cell signaling technology (Boston, USA).
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