Rat The ADM (molecular formula: C242H381N77O75S5) was obtained from Bachem (Bubendorff, Switzerland). PA was purchased from Sigma-Aldrich. ADM receptor antagonist ADM22-52 and Akt inhibitor A6730 were purchased from Anaspec (Fremont, CA, USA). DMEM, 0.25% trypsin-EDTA, fetal bovine serum, trypsin, and streptomycin/penicillin were from Thermo Fisher Scientific (Pudong New District, Shanghai, China). The antibodies of ADM, CRLR, RAMP2, RAMP3, and TNF-α were obtained from Affinity Biosciences (Pottstown, PA, USA). The antibodies of IL-6, IL-1β, SOD2, GPx1, CAT, and GAPDH were from Proteintech (SANYING, Wuhan, China). Total Akt and phosphorylated Akt were purchased from Cell Signaling Technology (Shanghai, China).
Adm22 52
Manufactured by AnaSpec
Sourced in United States
The ADM22-52 is a lab equipment product. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Trypsin, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Il 1β, by Proteintech (2 mentions)
Ramp2, by Affinity Biosciences (2 mentions)
Ramp3, by Affinity Biosciences (2 mentions)
Tnf α, by Affinity Biosciences (2 mentions)
Gapdh, by Proteintech (2 mentions)
Interleukin 6 (il 6), by Proteintech (1 mentions)
Total akt, by Cell Signaling Technology (1 mentions)
Phosphorylated akt, by Cell Signaling Technology (1 mentions)
α actin, by Abcam (1 mentions)
Interleukin 6 (il 6), by Affinity Biosciences (1 mentions)
Compound c, by Merck Group (1 mentions)
Inos antibody, by BD (1 mentions)
Total nf κb p65, by Cell Signaling Technology (1 mentions)
Rosiglitazone, by Merck Group (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
4 protocols using adm22 52
1
ADM Receptor Antagonist and Akt Inhibitor Effects
2
Adrenomedullin Regulation in Cell Culture
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Rat ADM (molecular formula: C242H381N77O75S5) was obtained from Bachem (Bubendorff, Switzerland). PA and Compound C were purchased from Sigma Aldrich (St. Louis, MO, USA). ADM22-52 was purchased from Anaspec (Fremont, CA, USA). DMEM, 0.25% trypsin-EDTA, fetal bovine serum, trypsin and streptomycin/penicillin were from Thermo Fisher Scientific (Waltham, MA, USA). The primary antibodies against α-actin and Runx2 were purchased from Abcam (Burlingame, CA, USA). ADM, CRLR, RAMP2, RAMP3, TNF-α and IL6 were obtained from Affinity Biosciences (Pottstown, PA, USA). The antibodies of IL-1β, NOX2, NOX4 and GAPDH were from Proteintech (SANYING, Wuhan, China).
3
Adrenomedullin Protocol for Inflammation
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ADM (molecular formula: C₂₄₂H₃₈₁N₇₇O₇₅S₅; sequence: H‐Tyr‐Arg‐Gln‐Ser‐Met‐Asn‐Gln‐Gly‐Ser‐Arg‐Ser‐Thr‐Gly‐Cys‐Arg‐Phe‐Gly‐Thr‐Cys‐Thr‐Met‐Gln‐Lys‐Leu‐Ala‐His‐Gln‐Ile‐Tyr‐Gln‐Phe‐Thr‐Asp‐Lys‐Asp‐Lys‐Asp‐Gly‐Met‐Ala‐Pro‐Arg‐Asn‐Lys‐Ile‐Ser‐Pro‐Gln‐Gly‐Tyr‐NH2) was purchased from Bachem (Bubendorff, Switzerland). IBMX, dexamethasone, rosiglitazone, and insulin were from Sigma‐Aldrich. LPS, PKA inhibitor P9115, and ADM receptor antagonist ADM22‐52 were from Anaspec (Fremont, California). DMEM, FBS, 0.25% trypsin‐EDTA, streptomycin/penicillin, and trypsin were obtained from Thermo Fisher Scientific (Pudong New District, Shanghai, China). The ADM, CRLR, RAMP2/3, and TNFα antibodies were from Abcam (Cambridge, UK). The IL‐1β antibody was obtained from Proteintech (SANYING, Wuhan, China). The COX‐2 antibody was from Cayman Chemical (AmyJet Scientific Inc., Hongshan, Wuhan, China). The iNOS antibody was purchased from BD Biosciences (Pudong New District, Shanghai, China). The phosphor‐IκBα, total‐IκBα, phosphor‐NF‐κB p65, total‐NF‐κB p65, phospho‐p38, total‐p38 MAPK, phospho‐ERK1/2, total‐ERK1/2, phospho‐JNK, and total‐JNK antibodies were obtained from Cell Signaling Technology (Shanghai) Biological Reagents, Inc. (Pudong New District, Shanghai, China).
4
Murine Models of Pulmonary Inflammation
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For papain-induced type 2 inflammation in the lung, mice were anesthetized with isoflurane, and then 25 μg of papain or PBS in a total volume of 40 μl was given i.n. on days 1, 2, and 3. BAL fluid and lungs were harvested and analyzed on day 4. For animal model of recombinant ADM treatment, mice were anesthetized with isoflurane, and 25 μg of recombinant ADM (Anaspec), or 100 μg of ADM22–52 (Anaspec) in a total volume of 40 μl was administered i.n. at indicated time points. Inflammatory cells in the lung were assessed on day 4. For helminth infection, third-stage larvae (L3) of N.b., which was obtained from Dr. D. Artis (Weill Cornell Medicine, New York, NY), were purified with a Baermann technique (Camberis et al., 2003 (link)). After washing three times in PBS, live worms were counted. On day 0, 300 or 500 purified worms were injected subcutaneously in 250 μl PBS. Mice were euthanized at the indicated time points to collect tissues for analysis or to count worm burden. For induction of bleomycin-induced pulmonary inflammation, mice were anesthetized with isoflurane and treated with 0.1 U bleomycin (Sigma-Aldrich) or PBS in a volume of 40 μl intratracheally, followed by analysis on day 7 to assess pulmonary inflammation.
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