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2kx2k multiport readout post column ccd

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The 2Kx2K multiport readout post column CCD is a laboratory instrument designed for high-resolution imaging and data acquisition. It features a 2048x2048 pixel CCD sensor with a multiport readout, allowing for rapid data capture and processing. The device is intended for use in scientific and research applications that require high-quality, high-speed image acquisition.

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Lab products found in correlation

Tecnai g2 spirit biotwin tem, by Thermo Fisher Scientific (3 mentions) Cf400 cu, by Electron Microscopy Sciences (1 mentions) Fei tecnaig2 spirit transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Tecnai g2 spirit transmission electron microscope, by Thermo Fisher Scientific (1 mentions)

4 protocols using 2kx2k multiport readout post column ccd

1

TEM Imaging of Biomolecular Samples

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The TEM experiments were performed similar to as described previously3 (link). Briefly, an aliquot of 5 μl sample was loaded onto a glow-discharged Formvar-coated 300-mesh copper grids for 30 s and was blotted by filter paper followed by washing the grid with 5 μl ddH2O. After another 30 s, 2% uranyl acetate was loaded on the grids and blotted again. The grid was dried for 1 min and loaded into a FEI Tecnai G2 Spirit Biotwin TEM. All images were captured using a Gatan 2Kx2K multiport readout post column CCD at the UT Southwestern EM Core Facility.
Hou Z., Chen D., Ryder B.D, & Joachimiak L.A. (2021). Biophysical properties of a tau seed. Scientific Reports, 11, 13602.
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2

Negative Staining TEM Imaging

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An aliquot of 5 μL sample was loaded onto a glow-discharged Formvar-coated 200-mesh copper grids for 30 s and was blotted by filter paper followed by washing the grid with 5 μL ddH2O. After another 30 s, 2% uranyl acetate was loaded on the grids and blotted again. The grid was dried for 1 min and loaded into an FEI Tecnai G2 Spirit Biotwin TEM. All images were captured using a Gatan 2Kx2K multiport readout post-column CCD at the UT Southwestern EM Core Facility.
Mullapudi V., Vaquer-Alicea J., Bommareddy V., Vega A.R., Ryder B.D., White CL I.I.I., Diamond M.I, & Joachimiak L.A. (2023). Network of hotspot interactions cluster tau amyloid folds. Nature Communications, 14, 895.
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3

Negative Staining Transmission Electron Microscopy

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5 μL sample was loaded onto a glow-discharged Formvar-coated 300-mesh copper grids for 1 minute and was blotted by filter paper followed by washing the grid with 5 μL ddH2O. After another 30 seconds, 2% uranyl acetate was loaded on the grids for 1 minute and blotted again. The grid was dried and loaded into a FEI Tecnai G2 Spirit Biotwin TEM. All images were captured using a Gatan 2Kx2K multiport readout post column CCD at the UT Southwestern EM Core Facility.
Li L., Nguyen B., Mullapudi V., Saelices L, & Joachimiak L.A. (2023). Disease-associated patterns of acetylation stabilize tau fibril formation. bioRxiv.
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4

Liposome Characterization by Transmission Electron Microscopy

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The electron microscopy of the liposomes was carried out on a FEI Tecnai G2 spirit transmission electron microscope. Sample (2.0 μL) was loaded to a glow-discharged carbon-coated copper grid (CF-400-Cu, Electron Microscopy Science, Hatfield, PA, USA). After 30 s incubation, the grid was blotted with a piece of Whatman No. 4 filter paper (Sigma-Aldrich, Co., St Louis, MO, USA) to remove excessive solution, then incubated with ammonium molybdate (6.0 %) (Sigma-Aldrich, Co., St Louis, MO, USA) adjusted pH with KOH to 6.4 for 30 s. After removing excessive staining solution, the stained grid was dried in air and then observed under FEI Tecnai G2 spirit transmission electron microscope (Hillsboro, OR, USA). Images were taken with a Gatan 2Kx2K multiport readout post column CCD (Pleasanton, CA, USA) at −2 to −3 μm defocus.
Yang L., Zheng H., Ratnakar J.S., Adebesin B.Y., Do Q.N., Kovacs Z, & Blount P. (2018). Engineering a pH-sensitive liposomal MRI agent by modification of a bacterial channel. Small (Weinheim an der Bergstrasse, Germany), 14(19), e1704256.
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