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Pre heated tissue culture plates

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Pre-heated tissue culture plates are a type of laboratory equipment designed to maintain a consistent temperature for cell culture applications. These plates are pre-warmed to a specific temperature, typically 37°C, to create an optimal environment for cell growth and maintenance.

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Lab products found in correlation

Rmil 7, by R&D Systems (2 mentions) Rhil 2, by Merck Group (2 mentions) Il 15, by R&D Systems (2 mentions)

2 protocols using pre heated tissue culture plates

1

Culturing Murine ILC1s with Crypts

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Approximately 1500-2500 murine ILC1 were seeded with ~100 mechanically disrupted SIO crypts per well, resuspended in 30μl ice-cold Matrigel, pipetted onto pre-heated tissue culture plates (Nunclon) and incubated at 37°C for 15-20min prior to addition of pre-warmed basal media supplemented with 50mM B2ME (R&D), 20ng/ml rhIL-2 (Sigma), 20ng/ml rmIL-7 (R&D), and 1ng/ml IL-15 (R&D), with media changes every 2-4 days.
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
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2

Culturing Murine ILC1s with Crypts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Approximately 1500-2500 murine ILC1 were seeded with ~100 mechanically disrupted SIO crypts per well, resuspended in 30μl ice-cold Matrigel, pipetted onto pre-heated tissue culture plates (Nunclon) and incubated at 37°C for 15-20min prior to addition of pre-warmed basal media supplemented with 50mM B2ME (R&D), 20ng/ml rhIL-2 (Sigma), 20ng/ml rmIL-7 (R&D), and 1ng/ml IL-15 (R&D), with media changes every 2-4 days.
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
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