Alexa fluor 568 conjugated anti mouse igg secondary antibody

Lyophilized fibrinogen from human plasma and Millex syringe filter (0.22 μm) were purchased from EMD Millipore (Billerica, MA). Lyophilized thrombin from bovine plasma, calcium chloride, ε-aminocaproic acid (εACA), and Alcian Blue 8GX were purchased from Sigma-Aldrich (St. Louis, MO). Peptides were synthesized by University of Utah DNA/Peptide synthesis core facility. Spectra/Por 7 dialysis membrane (MWCO = 3.5 kDa) was purchased from Spectrum Laboratories (Rancho Dominguez, CA). Coomassie Brilliant Blue R-250 was purchased from Genlantis (San Diego, CA). Sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate (Sulfo-LC-SPDP) and DyLight 680 NHS-ester were purchased from Thermo Fisher Scientific (Newington, NH). Mini-PROTEAN TGX precast electrophoresis gel was purchased from Bio-Rad (Hercules, CA). TO-PRO-3 iodide, Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody and Alexa Fluor 568 conjugated anti-mouse IgG secondary antibody were purchased from Invitrogen (Carlsband, CA). Rabbit anti-aquaporin 5 (AQP5), rabbit anti-TMEM-16A, rabbit anti-PECAM-1, mouse anti-cytokeratin 7, mouse anti- β-tubulin III, rabbit anti-Ki67 and Picrosirius Red Stain Kit were purchased from Abcam (Cambridge, MA). Mouse Na⁺/K⁺-ATPase antibody was purchased Santa Cruz Biotechnology (Santa Cruz, CA).