Melatonin, SB203580 (p38 inhibitor), SP600125 (JNK inhibitor) and BAY 11-7082 (NF-kB inhibitor) were obtained from Sigma-Aldrich. IL-1β was obtained from PeproTech. TPCK (IKB-α proteolysis inhibitor) and U0126 (MEK1/2 inhibitor) were obtained from Calbiochem. C021 (CCR4 antagonist) and RS102895 (CCR2 antagonist) were obtained from R&D Systems. CAY10591 (SIRT1 activator) was obtained from Cayman Chemicals. EX527 (inhibitor activator) was obtained from Tocris Bioscience. Primary antibodies against for β-actin and VCAM-1 were obtained from Abcam. Primary antibodies against for p38, ICAM-1, p65, PKCδ, ERK2, SIRT1, JNK1/3, p-PKCδ and p-ERK were obtained from Santa Cruz Biotechnology. Primary antibodies against for p-p65, p-p38 and p-JNK were obtained from cell signaling technology. Primary antibodies against for α-tubulin were obtained from Sigma-Aldrich. Neutralizing antibodies against human CCL2/MCP-1 (MAB279) were obtained from R&D Systems. On-target smart pool siRNA against ICAM-1 and VCAM-1 or non-targeting control siRNA were obtained from Dharmacon.
Mab279
Manufactured by R&D Systems
MAB279 is a mouse monoclonal antibody that recognizes human Endothelin-1. It can be used for the detection of Endothelin-1 in various immunoassay formats.
Automatically generated - may contain errors
Lab products found in correlation
Bay11 7082, by Merck Group (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Sp600125, by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
Melatonin, by Merck Group (1 mentions)
Rs102895, by R&D Systems (1 mentions)
Non targeting control sirna, by Horizon Discovery (1 mentions)
Ex527, by Bio-Techne (1 mentions)
Cay10591, by Cayman Chemical (1 mentions)
Mab002, by R&D Systems (1 mentions)
2 protocols using mab279
1
Mechanistic Insights into Inflammatory Signaling
2
Orthotopic Tumor Implantation and Anti-CCL2 Antibody
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Osmotic pumps were purchased from ALZET (Model 2004), with a manufacture pump rate of 0.23 μl per hour over 4 weeks. Osmotic pumps were filled with 1 mg/ml monoclonal mouse anti-human CCL2 antibody (R&D system, MAB279) or mouse IgG1 isotype control antibody (R&D System, MAB002) according to manufacturer's instructions. The filled pumps were equilibrated for 48 hours by incubation in 0.9% saline at 37°C. On the day of implantation, mice were anesthetized with 2% isoflurane. A 1-cm incision was made in the right dorsum, and one pump containing IgG or anti-CCL2 was inserted in each mouse (n = 5 per group). Pumps were implanted immediately after orthotopic transplantation of tumor cells. The wound was closed by wound clips. Wound clips were removed 7-10 days after surgery.
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