The sunlight power density (24 mW cm-2) was recorded at midday on 19 October, 2016, Strasbourg region, using a Handheld Laser Power Meter, 1917-R and Semrock band-pass filter 527 nm (50 nm bandwidth). The artificial white light mimicking sunlight was provided by a Cold light source from Zeiss, type KL 1500 LCD. The sample was illuminated from the top ∼2 cm from the divergent light source output through the same 527-nm filter, which corresponded to 15 mW cm-2 power density at the sample (light power divided by the illumination spot size of ∼1 cm2). Single-molecule imaging was done using a Nikon Ti-E inverted microscope using CFI Plan Apo 20X air (NA = 0.75) and CFI Plan Apo 60X oil (NA = 1.4) objectives and a Hamamatsu Orca Flash 4 camera. Donor channel was recorded through a 600 nm band-pass filter (50 nm bandwidth, Semrock), while the acceptor channel used a 647 nm long-pass filter (Semrock). Data were recorded and analysed using NIS Elements and Fiji software, respectively. The photo of setup is provided in the supplementary information (Supplementary Fig. 9 ).
Cfi plan apo 20x air
Manufactured by Hamamatsu Photonics
The CFI Plan Apo 20X air is a high-performance optical lens designed for microscopy applications. It provides a wide field of view and high numerical aperture, delivering excellent image quality and resolution. The lens is optimized for use in air and is a part of Hamamatsu Photonics' CFI Plan Apo series.
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2 protocols using cfi plan apo 20x air
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Single-Molecule Imaging of Photobiological Processes
2
Single-Molecule Imaging of Photobiological Processes
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The sunlight power density (24 mW cm-2) was recorded at midday on 19 October, 2016, Strasbourg region, using a Handheld Laser Power Meter, 1917-R and Semrock band-pass filter 527 nm (50 nm bandwidth). The artificial white light mimicking sunlight was provided by a Cold light source from Zeiss, type KL 1500 LCD. The sample was illuminated from the top ∼2 cm from the divergent light source output through the same 527-nm filter, which corresponded to 15 mW cm-2 power density at the sample (light power divided by the illumination spot size of ∼1 cm2). Single-molecule imaging was done using a Nikon Ti-E inverted microscope using CFI Plan Apo 20X air (NA = 0.75) and CFI Plan Apo 60X oil (NA = 1.4) objectives and a Hamamatsu Orca Flash 4 camera. Donor channel was recorded through a 600 nm band-pass filter (50 nm bandwidth, Semrock), while the acceptor channel used a 647 nm long-pass filter (Semrock). Data were recorded and analysed using NIS Elements and Fiji software, respectively. The photo of setup is provided in the supplementary information (Supplementary Fig. 9 ).
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