Log In Sign up
The largest database of trusted experimental protocols

Rpmi 1640 media

Manufactured by Cambrex
Visit Supplier

RPMI 1640 media is a cell culture medium commonly used for the in vitro cultivation of a variety of cell types, including lymphocytes, hybridomas, and other suspension cell lines. It provides a balanced salt solution and a source of essential nutrients to support cell growth and maintain cellular functions.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Hl 60 cells, by American Type Culture Collection (2 mentions) Iscove modified dulbecco media (imdm), by American Type Culture Collection (2 mentions) Sodium pyruvate, by Cambrex (2 mentions)

Spelling variants of this product

RPMI 1640 (13 citations)

2 protocols using rpmi 1640 media

1

Neutrophil-like Differentiation of HL-60 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HL-60 cells (CCL-240, ATCC, Manassas, VA) were cultured at 37°C in 5% CO2 in Iscove's Modified Dulbecco's Medium (IMDM, ATCC, Manassas, VA) containing 20% FBS (Sigma-Aldrich, St. Louis, MO) and differentiated to neutrophil-like cells by DMSO-induced differentiation for a period of 5 days. Cells were sub-cultured every third day to a density of 1×106 cells mL−1. During migration assays, neutrophils were suspended in a buffer of 0.2% human serum albumin (HSA, Sigma-Aldrich) in Hanks Buffered Salt Solution (HBSS, ATCC, Manassas,VA). T-lymphocytes were suspended in RPMI 1640 media (Cambrex, Charles City, IA) containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL−1 penicillin/streptomycin (Gibco-Invitrogen), 1% sodium bicarbonate and 1% sodium pyruvate (Cambrex).
Boneschansker L., Yan J., Wong E., Briscoe D.M, & Irimia D. (2014). MICROFLUIDIC PLATFORM FOR THE QUANTITATIVE ANALYSIS OF LEUKOCYTE MIGRATION SIGNATURES. Nature communications, 5, 4787.
+ Open protocol
+ Expand
2

Neutrophil-like Differentiation of HL-60 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HL-60 cells (CCL-240, ATCC, Manassas, VA) were cultured at 37°C in 5% CO2 in Iscove's Modified Dulbecco's Medium (IMDM, ATCC, Manassas, VA) containing 20% FBS (Sigma-Aldrich, St. Louis, MO) and differentiated to neutrophil-like cells by DMSO-induced differentiation for a period of 5 days. Cells were sub-cultured every third day to a density of 1×106 cells mL−1. During migration assays, neutrophils were suspended in a buffer of 0.2% human serum albumin (HSA, Sigma-Aldrich) in Hanks Buffered Salt Solution (HBSS, ATCC, Manassas,VA). T-lymphocytes were suspended in RPMI 1640 media (Cambrex, Charles City, IA) containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL−1 penicillin/streptomycin (Gibco-Invitrogen), 1% sodium bicarbonate and 1% sodium pyruvate (Cambrex).
Boneschansker L., Yan J., Wong E., Briscoe D.M, & Irimia D. (2014). MICROFLUIDIC PLATFORM FOR THE QUANTITATIVE ANALYSIS OF LEUKOCYTE MIGRATION SIGNATURES. Nature communications, 5, 4787.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!