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Pri mir200c

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Pri-miR200c is a laboratory product used for the study of microRNA (miRNA) biogenesis and regulation. It serves as a precursor to the mature miRNA-200c, which is involved in various cellular processes. The product enables researchers to investigate the production and function of this specific miRNA.

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Lab products found in correlation

Tri reagent, by Merck Group (2 mentions) Actinomycin d, by Merck Group (2 mentions) Click it nascent rna capture kit, by Thermo Fisher Scientific (2 mentions) Hsa mir200c, by Thermo Fisher Scientific (2 mentions) Pri mir200a, by Thermo Fisher Scientific (2 mentions) Pri mir200b, by Thermo Fisher Scientific (2 mentions) Pri mir429, by Thermo Fisher Scientific (2 mentions) Hsa mir200a, by Thermo Fisher Scientific (2 mentions) Hsa mir200b, by Thermo Fisher Scientific (2 mentions) Hsa mir429, by Thermo Fisher Scientific (2 mentions)

2 protocols using pri mir200c

1

Quantification of miRNA Biogenesis and Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated using TRI reagent (Sigma) and samples were reverse transcribed using qScript cDNA SuperMix (Quantas). Quantitative real-time PCR was performed on an Eppendorf Realplex2 (link) mastercycler using SYBR green (Quantas). Specific primers used are listed in Supplementary Table 2. For determination of DICER mRNA half life cells were treated with 5 μg/ml actinomycin D (Sigma) for the indicated times. De novo RNA synthesis was measured using the Click-iT Nascent RNA Capture Kit (Molecular Probes). Briefly, MCF7 and HMLER cells were pulse labeled with 0.2mM 5-ethynyl Uridine for 1 hour during aerobic conditions or 24 hrs hypoxia (0.2% O2) after which RNA was isolated as described above. Nascent RNA was captured using magnetic streptavidin beads, reverse transcribed and analyzed by quantitative real-time PCR. For determination of pri-miRNA and mature miRNA levels the following assays from Applied Biosystems were used: pri-miR200a (Hs03303376_pri), pri-miR200b (Hs03303027_pri), pri-miR429 (Hs03303727_pri), pri-miR200c (Hs03303157_pri), pri-miR141 (Hs03303157_pri), pri-miR210 (Hs03302948_pri), hsa-miR200a (000502), hsa-miR200b (002251), hsa-miR429 (001024), hsa-miR200c (002300), miR141 (002145), hsa-miR103 (000439), hsa-miR107 (000443), hsa-miR210 (000512), RNU44 (001094), RNU48 (001006).
van den Beucken T., Koch E., Chu K., Rupaimoole R., Prickaerts P., Adriaens M., Voncken J.W., Harris A.L., Buffa F.M., Haider S., Starmans M.H., Yao C.Q., Ivan M., Ivan C., Pecot C.V., Boutros P.C., Sood A.K., Koritzinsky M, & Wouters B.G. (2014). Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER. Nature communications, 5, 5203.
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2

Quantification of miRNA Biogenesis and Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was isolated using TRI reagent (Sigma) and samples were reverse transcribed using qScript cDNA SuperMix (Quantas). Quantitative real-time PCR was performed on an Eppendorf Realplex2 (link) mastercycler using SYBR green (Quantas). Specific primers used are listed in Supplementary Table 2. For determination of DICER mRNA half life cells were treated with 5 μg/ml actinomycin D (Sigma) for the indicated times. De novo RNA synthesis was measured using the Click-iT Nascent RNA Capture Kit (Molecular Probes). Briefly, MCF7 and HMLER cells were pulse labeled with 0.2mM 5-ethynyl Uridine for 1 hour during aerobic conditions or 24 hrs hypoxia (0.2% O2) after which RNA was isolated as described above. Nascent RNA was captured using magnetic streptavidin beads, reverse transcribed and analyzed by quantitative real-time PCR. For determination of pri-miRNA and mature miRNA levels the following assays from Applied Biosystems were used: pri-miR200a (Hs03303376_pri), pri-miR200b (Hs03303027_pri), pri-miR429 (Hs03303727_pri), pri-miR200c (Hs03303157_pri), pri-miR141 (Hs03303157_pri), pri-miR210 (Hs03302948_pri), hsa-miR200a (000502), hsa-miR200b (002251), hsa-miR429 (001024), hsa-miR200c (002300), miR141 (002145), hsa-miR103 (000439), hsa-miR107 (000443), hsa-miR210 (000512), RNU44 (001094), RNU48 (001006).
van den Beucken T., Koch E., Chu K., Rupaimoole R., Prickaerts P., Adriaens M., Voncken J.W., Harris A.L., Buffa F.M., Haider S., Starmans M.H., Yao C.Q., Ivan M., Ivan C., Pecot C.V., Boutros P.C., Sood A.K., Koritzinsky M, & Wouters B.G. (2014). Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER. Nature communications, 5, 5203.
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