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Sc 137006

Manufactured by Santa Cruz Biotechnology
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Sc-137006 is a laboratory equipment item produced by Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to facilitate standard laboratory procedures and experiments.

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Lab products found in correlation

Gapdh, by Merck Group (1 mentions) Histone h3, by Abcam (1 mentions) Ea0375box, by Thermo Fisher Scientific (1 mentions) Hdac3, by Santa Cruz Biotechnology (1 mentions) Dab staining kit, by ZSGB-BIO (1 mentions)

2 protocols using sc 137006

1

Western Blot Analysis of Neuronal Proteins

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Whole-cell or nuclear extracts from primary neurons or brain tissue were resolved on 3–8% Tris-Acetate gels (Thermo Fisher, EA0375BOX) or 10% Tris-Glycine gels and transferred to nitrocelluose. Membranes were incubated overnight in the following primary antibodies: NPAS3 (Gift of Steven McKnight), NCoR2 (Thermo Fisher PA1–843 (Ab1); Millipore, 06891 (Ab2)), TBL1 (Abcam 24528 (Ab1); Santa Cruz (H-3): sc-137006 (Ab2)), HDAC3 (Santa Cruz sc11416X), β-TUBULIN3 (Covance), GAPDH (Sigma G9545), MYC (EMD Millipore 05–724, Developmental Studies Hybridoma Bank 9E10), FOS (Santa Cruz, sc7202X), Histone H3 (Abcam, ab1791). Following washing, membranes were incubated with secondary antibodies conjugated to IRdye 700 or 800 and imaged with LiCOR Odyssey.
Sharma N., Pollina E.A., Nagy M.A., Yap E.L., DiBiase F.A., Hrvatin S., Hu L., Lin C, & Greenberg M.E. (2019). ARNT2 tunes activity-dependent gene expression through NCoR2-mediated repression and NPAS4-mediated activation. Neuron, 102(2), 390-406.e9.
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2

Immunohistochemical Staining of NPC Tissues

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The paraffin-embedded NPC tissues were sectioned into 5 µm-thick slides. The slides were deparaffinized, performed for antigen retrieval, and blocked with a proper blocking solution. The slides were then incubated with the primary antibody against TBL1X (1:50, #sc137006, Santacruz) or Flot2 (1:50, #28208-1-AP, Proteintech). After that, the slides were incubated with relative secondary antibodies and stained with a DAB staining kit (#ZLI9017, ZSGB BIO, Beijing, China).
Xu H., Yan X., Zhu H., Kang Y., Luo W., Zhao J., Zhou K., Liu X., Ye L., Zhou Q., Li S., Zhao M., Wang L., Zhu B., Liu W., Li J., Jiang X, & Ren C. (2022). TBL1X and Flot2 form a positive feedback loop to promote metastasis in nasopharyngeal carcinoma. International Journal of Biological Sciences, 18(3), 1134-1149.
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