DNA fragments were analyzed on denaturing polyacrylamide gel electrophoresis using 6% Novex TBE Urea gel (Thermo Fisher Scientific, Waltham, MA). After electrophoresis, the gel was stained with SYBR Gold gel stain (Thermo Fisher Scientific) and photographed with ChemiDoc Touch (Bio Rad Laboratories, Hercules, CA). Quantitation was performed using Image Lab 5.2 (Bio Rad Laboratories).
Sybr gold gel stain
Manufactured by Thermo Fisher Scientific
Sourced in United States
SYBR Gold gel stain is a nucleic acid stain used for the detection of DNA and RNA in agarose and polyacrylamide gels. It exhibits enhanced fluorescence upon binding to nucleic acids and can be visualized using a UV or blue-light transilluminator.
Automatically generated - may contain errors
Lab products found in correlation
Image lab 5, by Bio-Rad (1 mentions)
Chemidoc touch, by Bio-Rad (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
Ultrapure agarose, by Thermo Fisher Scientific (1 mentions)
Efluor 450, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Non essential amino acids (neaa), by Avantor (1 mentions)
Ligation buffer, by Thermo Fisher Scientific (1 mentions)
Loading buffer, by New England Biolabs (1 mentions)
Tris hcl buffer, by Thermo Fisher Scientific (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Thermo Fisher Scientific (1 mentions)
Agarose, by Merck Group (1 mentions)
Generuler low range dna ladder, by Thermo Fisher Scientific (1 mentions)
3 protocols using sybr gold gel stain
1
Denaturing Gel Electrophoresis of DNA Fragments
2
MOG-R3 Peptide Synthesis and Characterization
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MOG-R3 peptide (MEVGWYRSPFSRVVLHLYRNGKRRR) was synthesized by Genscript at > 95%purity (Piscataway, NJ). CpG DNA (5’-T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T*T*-3’) and GpG DNA (5’-T*G*A*C*T*G*T*G*A*A*G*G*T*T*A*G*A*G*A*T*G*A*-3’) was purchased from Integrated DNA Technology (Coralville, IA). β-mercaptoethanol, ethylenediaminetetraacetic acid (EDTA), 5x tris-borate-EDTA (TBE) buffer, and bovine serum albumin (BSA) were purchased from Sigma Aldrich (St. Louis, MO). Molecular biology grade water, fetal bovine serum (FBS), 20X phosphate buffered solution (PBS), 20% sodium dodecyl sulfate (SDS), HEPES, and non-essential amino acids were purchased from VWR (Radnor, PA). RPMI-1640 media, SYBR Gold gel stain, l-glutamine, penicillin-streptomycin, Ultrapure Agarose, Brilliant Violet 450 viability stain, and cell proliferation dye eFluor450 were purchased from Thermo Fisher Scientific (Grand Island, NY).
3
Molecular Diagnostics Assay Development
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T4 DNA ligase, 10× ligation buffer, bovine serum albumin (BSA), ATP, GeneRuler low range DNA ladder, SYBR Gold gel stain, Tris-HCl buffer (1 M, pH 8.0), and Tris-acetate-EDTA buffer (TAE, 50×) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Bst 3.0 polymerase, Bst buffer (isothermal amplification buffer II, 10×), MgSO 4 , Nb.BtsI nickase, and loading buffer were obtained from New England BioLabs (Ipswich, MA, USA). Fetal bovine serum (FBS) and agarose were obtained from Sigma-Aldrich (St. Louis, MO, USA). Streptavidin-coated cross-linked hydroxyethyl starch iron oxide composite particles (100 nm size MNP, product code 10-19-102) were supplied by Micromod Partikeltechnologie GmbH (Rostock, Germany). Sequences of target, PLP, and detection probes listed in Table S1 were synthesized by Integrated DNA Technologies (Coralville, IA, USA) and dissolved in 50 mM Tris-HCl (pH 8.0).
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